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Establishment of new method for analysis of starch contents and varietal differences in soybean seeds

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Title: Establishment of new method for analysis of starch contents and varietal differences in soybean seeds
Authors: Jeong, Woo-Hyeun Browse this author
Harada, Kyuya Browse this author
Yamada, Tetsuya Browse this author →KAKEN DB
Abe, Jun Browse this author
Kitamura, Keisuke Browse this author
Keywords: starch content
heat-resistant α-amylase
dinitrosalicylic acid
Issue Date: 2010
Publisher: Japanese Society of Breeding
Journal Title: Breeding Science
Volume: 60
Issue: 2
Start Page: 160
End Page: 163
Publisher DOI: 10.1270/jsbbs.60.160
Abstract: Aside from a report claiming that soybean seeds contain less than 1% starch, little is actually known about the genetic variation of the starch content in this important crop species. We used a starch-iodine test to identify varieties with high starch content in a soybean germplasm collection and found a total of 34 cultivars that showed a strongly positive reaction (dark color). For a more accurate quantitation of starch contents, we established a new method using a heat-resistant α-amylase and dinitrosalicylic acid (DNS) reagent. We compared the DNS method and the standard method using glucose-oxidase (GOD) with a known amount of starch as a standard. We showed that the DNS method generated results that were very highly correlated with the GOD method. In addition, we found the new method to be easier and faster to implement than the GOD method. Using the DNS method, we found several accessions in our soybean germplasm whose starch contents were 2-7%.
Type: article
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 山田 哲也

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