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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Agriculture / Faculty of Agriculture >
Peer-reviewed Journal Articles, etc >
Establishment of new method for analysis of starch contents and varietal differences in soybean seeds
| Title: | Establishment of new method for analysis of starch contents and varietal differences in soybean seeds |
| Authors: | Jeong, Woo-Hyeun Browse this author | | Harada, Kyuya Browse this author | | Yamada, Tetsuya Browse this author →KAKEN DB | | Abe, Jun Browse this author | | Kitamura, Keisuke Browse this author |
| Keywords: | starch content | | soybean | | iodine-starch | | heat-resistant α-amylase | | dinitrosalicylic acid |
| Issue Date: | 2010 |
| Publisher: | Japanese Society of Breeding |
| Journal Title: | Breeding Science |
| Volume: | 60 |
| Issue: | 2 |
| Start Page: | 160 |
| End Page: | 163 |
| Publisher DOI: | 10.1270/jsbbs.60.160 |
| Abstract: | Aside from a report claiming that soybean seeds contain less than 1% starch, little is actually known about the genetic variation of the starch content in this important crop species. We used a starch-iodine test to identify varieties with high starch content in a soybean germplasm collection and found a total of 34 cultivars that showed a strongly positive reaction (dark color). For a more accurate quantitation of starch contents, we established a new method using a heat-resistant α-amylase and dinitrosalicylic acid (DNS) reagent. We compared the DNS method and the standard method using glucose-oxidase (GOD) with a known amount of starch as a standard. We showed that the DNS method generated results that were very highly correlated with the GOD method. In addition, we found the new method to be easier and faster to implement than the GOD method. Using the DNS method, we found several accessions in our soybean germplasm whose starch contents were 2-7%. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/47515 |
| Appears in Collections: | 農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 山田 哲也
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