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Enhancement of femtosecond laser-induced nucleation of protein in a gel solution

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Title: Enhancement of femtosecond laser-induced nucleation of protein in a gel solution
Authors: Murai, Ryota Browse this author
Yoshikawa, Hiroshi Y. Browse this author
Takahashi, Yoshinori Browse this author
Maruyama, Mihoko Browse this author
Sugiyama, Shigeru Browse this author
Sazaki, Gen Browse this author →KAKEN DB
Adachi, Hiroaki Browse this author
Takano, Kazufumi Browse this author
Matsumura, Hiroyoshi Browse this author
Murakami, Satoshi Browse this author
Inoue, Tsuyoshi Browse this author
Mori, Yusuke Browse this author
Keywords: biodiffusion
biomolecular effects of radiation
bubbles
cavitation
enzymes
fluorescence
gels
nucleation
Issue Date: 25-Jan-2010
Publisher: American Institute of Physics
Journal Title: Applied Physics Letters
Volume: 96
Issue: 4
Start Page: 043702
Publisher DOI: 10.1063/1.3294622
Abstract: We found that the use of a gel solution with agarose enhanced femtosecond laser-induced nucleation and produced hen egg white lysozyme crystals at three to five times lower supersaturation than those by the femtosecond laser or agarose alone. The fast fluorescence imaging of the protein in the gel solution revealed that cavitation bubbles created high-concentration regions at the focal point, which could be the trigger for protein nucleation. The lower diffusions of protein molecules in agarose gel retained the high-concentration regions for a longer time, and facilitated the nucleation.
Rights: Copyright 2010 American Institute of Physics. This article may be downloaded for personal use only. Any other use requires prior permission of the author and the American Institute of Physics. The following article appeared in Appl. Phys. Lett. 96, 043702 (2010) and may be found at https://dx.doi.org/10.1063/1.3294622
Type: article
URI: http://hdl.handle.net/2115/47520
Appears in Collections:低温科学研究所 (Institute of Low Temperature Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 佐崎 元

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