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High expression of the mRNA of cytochrome P450 and phase II enzymes in the lung and kidney tissues of cattle

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Title: High expression of the mRNA of cytochrome P450 and phase II enzymes in the lung and kidney tissues of cattle
Authors: Darwish, W. S. Browse this author
Ikenaka, Y. Browse this author
El-Ghareeb, W. R. Browse this author
Ishizuka, M. Browse this author →KAKEN DB
Keywords: cytochrome P450
phase II enzymes
tissue distribution
Issue Date: 1-Dec-2010
Publisher: Cambridge University Press
Journal Title: Animal
Volume: 4
Issue: 12
Start Page: 2023
End Page: 2029
Publisher DOI: 10.1017/S1751731110001394
PMID: 22445377
Abstract: The objective of this study was to investigate the tissue-specific mRNA expression of different cytochrome P450 (CYP) isoforms, UDP glucuronsyl transferase 1A1 (UGT1A1) and glutathione-S-transferase (GSTA1) in the different tissues (liver, mammary gland, lungs, spleen, kidney cortex, heart, masseter muscle and tongue) of cattle, using quantitative real-time polymerase chain reaction (qPCR). CYP1A1-like mRNA was expressed in all of the tissues examined, including the liver, with the highest expression level in the kidney. CYP1A2-, 2E1- and 3A4-like mRNAs were only expressed hepatically. Interestingly, significant expression of CYP2B6-like mRNA was recorded in the lung tissue, while CYP2C9-like mRNA was expressed in the liver and kidney tissues of the cattle examined. UGT1A1- and GSTA1-like mRNAs were expressed in all of the examined tissues, except the mammary glands, and the highest expression levels were recorded in the kidney. The high expression of UGT1A1 in the lung tissue and GSTA1 in the liver tissue was unique to cattle; this has not been reported for rats or mice. The findings of this study strongly suggest that the liver, kidneys and lungs of cattle are the major organs contributing to xenobiotics metabolism.
Rights: © The Animal Consortium 2010
Type: article
Appears in Collections:獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 石塚 真由美

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