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Crystallization and preliminary crystallographic analysis of dextranase from Streptococcus mutans

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Title: Crystallization and preliminary crystallographic analysis of dextranase from Streptococcus mutans
Authors: Suzuki, Nobuhiro Browse this author
Kim, Young-Min Browse this author
Fujimoto, Zui Browse this author
Momma, Mitsuru Browse this author
Kang, Hee-Kwon Browse this author
Funane, Kazumi Browse this author
Okuyama, Masayuki Browse this author
Mori, Haruhide Browse this author
Kimura, Atsuo Browse this author →KAKEN DB
Keywords: dextran
dextranases
glycoside hydrolase family 66
isomaltooligosaccharides
Streptococcus mutans
Issue Date: Dec-2011
Publisher: International Union of Crystallography
Journal Title: Acta Crystallographica Section F: Structural Biology and Crystallization Communications
Volume: 67
Issue: 12
Start Page: 1542
End Page: 1544
Publisher DOI: 10.1107/S1744309111038425
Abstract: Streptococcus mutans dextranase hydrolyzes the internal α-1,6-linkages of dextran and belongs to glycoside hydrolase family 66. An N- and C-terminal deletion mutant of S. mutans dextranase was crystallized by the sitting-drop vapour-diffusion method. The crystals diffracted to a resolution of 1.6 Å and belonged to space group P2_[1], with unit-cell parameters a = 53.2, b = 89.7, c = 63.3 Å, β = 102.3°. Assuming that the asymmetric unit of the crystal contained one molecule, the Matthews coefficient was calculated to be 4.07 Å^[3] Da^[-1]; assuming the presence of two molecules in the asymmetric unit it was calculated to be 2.03 Å 3 Da^[-1].
Type: article
URI: http://hdl.handle.net/2115/47973
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 木村 淳夫

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