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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Agriculture / Faculty of Agriculture >
Peer-reviewed Journal Articles, etc >
Crystallization and preliminary crystallographic analysis of dextranase from Streptococcus mutans
| Title: | Crystallization and preliminary crystallographic analysis of dextranase from Streptococcus mutans |
| Authors: | Suzuki, Nobuhiro Browse this author | | Kim, Young-Min Browse this author | | Fujimoto, Zui Browse this author | | Momma, Mitsuru Browse this author | | Kang, Hee-Kwon Browse this author | | Funane, Kazumi Browse this author | | Okuyama, Masayuki Browse this author | | Mori, Haruhide Browse this author | | Kimura, Atsuo Browse this author →KAKEN DB |
| Keywords: | dextran | | dextranases | | glycoside hydrolase family 66 | | isomaltooligosaccharides | | Streptococcus mutans |
| Issue Date: | Dec-2011 |
| Publisher: | International Union of Crystallography |
| Journal Title: | Acta Crystallographica Section F: Structural Biology and Crystallization Communications |
| Volume: | 67 |
| Issue: | 12 |
| Start Page: | 1542 |
| End Page: | 1544 |
| Publisher DOI: | 10.1107/S1744309111038425 |
| Abstract: | Streptococcus mutans dextranase hydrolyzes the internal α-1,6-linkages of dextran and belongs to glycoside hydrolase family 66. An N- and C-terminal deletion mutant of S. mutans dextranase was crystallized by the sitting-drop vapour-diffusion method. The crystals diffracted to a resolution of 1.6 Å and belonged to space group P2_[1], with unit-cell parameters a = 53.2, b = 89.7, c = 63.3 Å, β = 102.3°. Assuming that the asymmetric unit of the crystal contained one molecule, the Matthews coefficient was calculated to be 4.07 Å^[3] Da^[-1]; assuming the presence of two molecules in the asymmetric unit it was calculated to be 2.03 Å 3 Da^[-1]. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/47973 |
| Appears in Collections: | 農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 木村 淳夫
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