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Interactions between canine RAD51 and full length or truncated BRCA2 BRC repeats
Title: | Interactions between canine RAD51 and full length or truncated BRCA2 BRC repeats |
Authors: | Ochiai, K. Browse this author | Yoshikawa, Y. Browse this author | Oonuma, T. Browse this author | Tomioka, Y. Browse this author | Hashizume, K. Browse this author | Morimatsu, M. Browse this author |
Keywords: | Canine | BRCA2 | RAD51 |
Issue Date: | Nov-2011 |
Publisher: | Elsevier |
Journal Title: | The Veterinary Journal |
Volume: | 190 |
Issue: | 2 |
Start Page: | 293 |
End Page: | 295 |
Publisher DOI: | 10.1016/j.tvjl.2010.11.001 |
Abstract: | In humans, mutations of the breast cancer susceptibility protein BRCA2 interact with recombinase RAD51 and increase the risk of cancer. This interaction occurs via a series of eight BRC repeat sequences in BRCA2. A mammalian two-hybrid assay using individual BRC repeats demonstrated that all the repeats except BRC6 bind RAD51 strongly (BRC1, 2 and 4), with intermediate strength (BRC8), or weakly (BRC3, 5 and 7). In serial deletion mutation experiments, the binding strengths were increased when the C-terminal BRC repeat was removed from BRC1-8, BRC1-5 and BRC1-3. These results provide an understanding of the basic function of canine BRCA2 and may be helpful to estimate the effect of missense or truncation mutations in canine mammary tumours. |
Type: | article (author version) |
URI: | http://hdl.handle.net/2115/47982 |
Appears in Collections: | 遺伝子病制御研究所 (Institute for Genetic Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 森松 正美
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