|
Hokkaido University Collection of Scholarly and Academic Papers >
Institute for Genetic Medicine >
Peer-reviewed Journal Articles, etc >
Interactions between canine RAD51 and full length or truncated BRCA2 BRC repeats
| Title: | Interactions between canine RAD51 and full length or truncated BRCA2 BRC repeats |
| Authors: | Ochiai, K. Browse this author | | Yoshikawa, Y. Browse this author | | Oonuma, T. Browse this author | | Tomioka, Y. Browse this author | | Hashizume, K. Browse this author | | Morimatsu, M. Browse this author |
| Keywords: | Canine | | BRCA2 | | RAD51 |
| Issue Date: | Nov-2011 |
| Publisher: | Elsevier |
| Journal Title: | The Veterinary Journal |
| Volume: | 190 |
| Issue: | 2 |
| Start Page: | 293 |
| End Page: | 295 |
| Publisher DOI: | 10.1016/j.tvjl.2010.11.001 |
| Abstract: | In humans, mutations of the breast cancer susceptibility protein BRCA2 interact with recombinase RAD51 and increase the risk of cancer. This interaction occurs via a series of eight BRC repeat sequences in BRCA2. A mammalian two-hybrid assay using individual BRC repeats demonstrated that all the repeats except BRC6 bind RAD51 strongly (BRC1, 2 and 4), with intermediate strength (BRC8), or weakly (BRC3, 5 and 7). In serial deletion mutation experiments, the binding strengths were increased when the C-terminal BRC repeat was removed from BRC1-8, BRC1-5 and BRC1-3. These results provide an understanding of the basic function of canine BRCA2 and may be helpful to estimate the effect of missense or truncation mutations in canine mammary tumours. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/47982 |
| Appears in Collections: | 遺伝子病制御研究所 (Institute for Genetic Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
Submitter: 森松 正美
|
