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Effect of calcium ion on the thermal denaturation of subfragment-1 and rod regions of squid myosin upon the heating of myofibrils
| Title: | Effect of calcium ion on the thermal denaturation of subfragment-1 and rod regions of squid myosin upon the heating of myofibrils |
| Other Titles: | スルメイカ筋原線維の加熱によるミオシン・ サブフラグメント-1およびロッド変性に対するCa2+の影響 |
| Authors: | Abe, Kazuyo1 Browse this author | | Kinoshita, Yasunori2 Browse this author | | Konno, Kunihiko3 Browse this author →KAKEN DB |
| Authors(alt): | 阿部, 一世1 | | 木下, 康宣2 | | 今野, 久仁彦3 |
| Keywords: | Squid | | Myosin | | Thermal denaturation | | Myofibril | | Calcium ion |
| Issue Date: | Jan-2012 |
| Publisher: | Springer Japan |
| Journal Title: | Fisheries Science |
| Volume: | 78 |
| Issue: | 1 |
| Start Page: | 163 |
| End Page: | 168 |
| Publisher DOI: | 10.1007/s12562-011-0435-7 |
| Abstract: | Thermal inactivation of Ca^[2+]-ATPase of squid myofibrils was significantly suppressed in the presence of Ca^[2+]. Monomeric myosin content decreased much faster than Ca^[2+]-ATPase inactivation in Ca-medium, which was well explained by the fast rod denaturation. In contrast, rod denaturation was slower than S-1 in EDTA medium. Decrease in monomeric myosin content was explained by faster S-1 denaturation. Comparing the S-1 and rod denaturation rates at a fixed temperature, it was concluded that S-1 denaturation was suppressed by Ca^[2+] whereas the rod portion was not. Unfolding experiment with isolated myosin rod confirmed no stabilizing effect of Ca2+ on rod. It was concluded that significant stabilization of S-1 portion by Ca2+ generated apparently different myosin denaturation pattern in two media. | | スルメイカ筋原線維のATPase失活はCa2+存在下で著しく抑制された。Ca2+存在下でのATPase失活より速い単量体ミオシンの減少は、速いロッドの変性で説明できた。一方、EDTA中では、単量体ミオシンの減少はRod変性より速いS-1の変性に対応した。同一温度でのS-1、Rod変性速度の比較から、Ca2+による安定化はS-1に限定されると推定した。これはRodの加熱に伴うへリックス崩壊にCa2+は影響を与えないことから確認した。それゆえ、イカミオシンの変性様式はCa2+によるS-1の大きな安定化により発現したと結論した。 |
| Rights: | © 2012 公益社団法人日本水産学会 | | The final publication is available at www.springerlink.com | | © 2012 The Japanese Society of Fisheries Science |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/48256 |
| Appears in Collections: | 水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 今野 久仁彦
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