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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Health Sciences / Faculty of Health Sciences >
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Amoebal Endosymbiont Protochlamydia Induces Apoptosis to Human Immortal HEp-2 Cells
This item is licensed under:Creative Commons Attribution 3.0 Unported
| Title: | Amoebal Endosymbiont Protochlamydia Induces Apoptosis to Human Immortal HEp-2 Cells |
| Authors: | Ito, Atsushi Browse this author | | Matsuo, Junji Browse this author →KAKEN DB | | Nakamura, Shinji Browse this author →KAKEN DB | | Yoshida, Asahi Browse this author | | Okude, Miho Browse this author | | Hayashi, Yasuhiro Browse this author →KAKEN DB | | Sakai, Haruna Browse this author | | Yoshida, Mitsutaka Browse this author | | Takahashi, Kaori Browse this author | | Yamaguchi, Hiroyuki Browse this author →KAKEN DB |
| Issue Date: | Jan-2012 |
| Publisher: | Public Library of Science |
| Journal Title: | PLoS ONE |
| Volume: | 7 |
| Issue: | 1 |
| Start Page: | e30270 |
| Publisher DOI: | 10.1371/journal.pone.0030270 |
| Abstract: | Protochlamydia, an environmental chlamydia and obligate amoebal endosymbiotic bacterium, evolved to survive within protist hosts, such as Acanthamobae, 700 million years ago. However, these bacteria do not live in vertebrates, including humans. This raises the possibility that interactions between Protochlamydia and human cells could induce a novel cytopathic effect, leading to new insights into host-parasite relationships. Therefore, we studied the effect of Protochlamydia on the survival of human immortal cell line, HEp-2 cells and primary peripheral blood mononuclear cells (PBMC). Using mainly 4′,6-diamidino-2-phenylindole staining, fluorescent in situ hybridization, transmission electron microscopy, and also TUNEL and Transwell assays, we demonstrated that the Protochlamydia induced apoptosis in HEp-2 cells. The attachment of viable bacterial cells, but not an increase of bacterial infectious progenies within the cells, was required for the apoptosis. Other chlamydiae [Parachlamydia acanthamoebae and Chlamydia trachomatis (serovars D and L2)] did not induce the same phenomena, indicating that the observed apoptosis may be specific to the Protochlamydia. Furthermore, the bacteria had no effect on the survival of primary PBMCs collected from five volunteers, regardless of activation. We concluded that Protochlamydia induces apoptosis in human-immortal HEp-2 cells and that this endosymbiont could potentially be used as a biological tool for the elucidation of novel host-parasite relationships. |
| Rights: | http://creativecommons.org/licenses/by/3.0/ |
| Relation: | http://www.plospathogens.org/static/license.action |
| Type: | article |
| URI: | http://hdl.handle.net/2115/48269 |
| Appears in Collections: | 保健科学院・保健科学研究院 (Graduate School of Health Sciences / Faculty of Health Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 山口 博之
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