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Amoebal Endosymbiont Protochlamydia Induces Apoptosis to Human Immortal HEp-2 Cells

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Title: Amoebal Endosymbiont Protochlamydia Induces Apoptosis to Human Immortal HEp-2 Cells
Authors: Ito, Atsushi Browse this author
Matsuo, Junji Browse this author →KAKEN DB
Nakamura, Shinji Browse this author →KAKEN DB
Yoshida, Asahi Browse this author
Okude, Miho Browse this author
Hayashi, Yasuhiro Browse this author →KAKEN DB
Sakai, Haruna Browse this author
Yoshida, Mitsutaka Browse this author
Takahashi, Kaori Browse this author
Yamaguchi, Hiroyuki Browse this author →KAKEN DB
Issue Date: Jan-2012
Publisher: Public Library of Science
Journal Title: PLoS ONE
Volume: 7
Issue: 1
Start Page: e30270
Publisher DOI: 10.1371/journal.pone.0030270
Abstract: Protochlamydia, an environmental chlamydia and obligate amoebal endosymbiotic bacterium, evolved to survive within protist hosts, such as Acanthamobae, 700 million years ago. However, these bacteria do not live in vertebrates, including humans. This raises the possibility that interactions between Protochlamydia and human cells could induce a novel cytopathic effect, leading to new insights into host-parasite relationships. Therefore, we studied the effect of Protochlamydia on the survival of human immortal cell line, HEp-2 cells and primary peripheral blood mononuclear cells (PBMC). Using mainly 4′,6-diamidino-2-phenylindole staining, fluorescent in situ hybridization, transmission electron microscopy, and also TUNEL and Transwell assays, we demonstrated that the Protochlamydia induced apoptosis in HEp-2 cells. The attachment of viable bacterial cells, but not an increase of bacterial infectious progenies within the cells, was required for the apoptosis. Other chlamydiae [Parachlamydia acanthamoebae and Chlamydia trachomatis (serovars D and L2)] did not induce the same phenomena, indicating that the observed apoptosis may be specific to the Protochlamydia. Furthermore, the bacteria had no effect on the survival of primary PBMCs collected from five volunteers, regardless of activation. We concluded that Protochlamydia induces apoptosis in human-immortal HEp-2 cells and that this endosymbiont could potentially be used as a biological tool for the elucidation of novel host-parasite relationships.
Type: article
Appears in Collections:保健科学院・保健科学研究院 (Graduate School of Health Sciences / Faculty of Health Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 山口 博之

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