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Truncation of N- and C-terminal regions of Streptococcus mutans dextranase enhances catalytic activity.

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Title: Truncation of N- and C-terminal regions of Streptococcus mutans dextranase enhances catalytic activity.
Authors: Kim, Young-Min Browse this author
Shimizu, Ryoko Browse this author
Nakai, Hiroyuki Browse this author
Mori, Haruhide Browse this author
Okuyama, Masayuki Browse this author
Kang, Min-Sun Browse this author
Fujimoto, Zui Browse this author
Funane, Kazumi Browse this author
Kim, Doman Browse this author
Kimura, Atsuo Browse this author →KAKEN DB
Keywords: endo-dextranase
glycoside hydrolase family 66
limited proteolysis
Issue Date: Jul-2011
Publisher: springer
Journal Title: Applied microbiology and biotechnology
Volume: 91
Issue: 2
Start Page: 329
End Page: 339
Publisher DOI: 10.1007/s00253-011-3201-y
PMID: 21479716
Abstract: Multiple forms of native and recombinant endo-dextranases (Dexs) of the glycoside hydrolase family (GH) 66 exist. The GH 66 Dex gene from Streptococcus mutans ATCC 25175 (SmDex) was expressed in Escherichia coli. The recombinant full-size (95.4 kDa) SmDex protein was digested to form an 89.8 kDa isoform (SmDex90). The purified SmDex90 was proteolytically degraded to more than seven polypeptides (23-70 kDa) during long storage. The protease-insensitive protein was desirable for the biochemical analysis and utilization of SmDex. GH 66 Dex was predicted to comprise four regions from the N- to C-termini: N-terminal variable region (N-VR), conserved region (CR), glucan-binding site (GBS), and C-terminal variable region (C-VR). Five truncated SmDexs were generated by deleting N-VR, GBS, and/or C-VR. Two truncation-mutant enzymes devoid of C-VR (TM-NCGΔ) or N-VR/C-VR (TM-ΔCGΔ) were catalytically active, thereby indicating that N-VR and C-VR were not essential for the catalytic activity. TM-ΔCGΔ did not accept any further protease-degradation during long storage. TM-NCGΔ and TM-ΔCGΔ enhanced substrate hydrolysis, suggesting that N-VR and C-VR induce hindered substrate binding to the active site.
Type: article (author version)
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 木村 淳夫

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