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Isolation of fucoxanthin and fatty acids analysis of Padina australis and cytotoxic effect of fucoxanthin on human lung cancer (H1299) cell lines
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| Title: | Isolation of fucoxanthin and fatty acids analysis of Padina australis and cytotoxic effect of fucoxanthin on human lung cancer (H1299) cell lines |
| Authors: | Jaswir, Irwandi Browse this author | | Noviendri, Dedi Browse this author | | Salleh, Hamzah Mohd. Browse this author | | Taher, Muhammad Browse this author | | Miyashita, Kazuo Browse this author →KAKEN DB |
| Keywords: | Brown seaweed | | Padina australis | | fucoxanthin | | fatty acid methyl ester | | H1299 cell lines | | human lung cancer | | MTT assay |
| Issue Date: | 16-Dec-2011 |
| Publisher: | Academic Journals |
| Journal Title: | African Journal of Biotechnology |
| Volume: | 10 |
| Issue: | 81 |
| Start Page: | 18855 |
| End Page: | 18862 |
| Publisher DOI: | 10.5897/AJB11.2765 |
| Abstract: | Fucoxanthin has been successfully isolated from species of Malaysian brown seaweed, namely Padina australis. The purity of the fucoxanthin is >98% as indicated by high performance liquid chromatography analysis. This seaweed also contains a considerable amount of unsaturated fatty acids. Thirteen fatty acids were detected with gas chromatography. However, fatty acid methyl ester (FAMEs) of eicosapentanoic acid (C20:5n-3), arachidonic acid (C20:4n-6), linoleic acid (C18:2n-6) and alpha-linolenic acid (C18:3n-3) contents of P. australis were found to be 2.06, 9.50, 6.37, and 2.83%, respectively. For saturated fatty acids, palmitic acid (C16:0) was found to be the major fatty acid with about 23.97%. Furthermore, data obtained from the methyl thiazolyl tetrazolium (MTT) assay indicated that fucoxanthin reduced the viability of H1299 cell lines, showing an IC50 value of 2.45 mM. |
| Rights: | http://creativecommons.org/licenses/by/3.0/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/48392 |
| Appears in Collections: | 水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 宮下 和夫
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