|
Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Medicine / Faculty of Medicine >
Peer-reviewed Journal Articles, etc >
DDX60, a DEXD/H Box Helicase, Is a Novel Antiviral Factor Promoting RIG-I-Like Receptor-Mediated Signaling
| Title: | DDX60, a DEXD/H Box Helicase, Is a Novel Antiviral Factor Promoting RIG-I-Like Receptor-Mediated Signaling |
| Authors: | Miyashita, Moeko Browse this author | | Oshiumi, Hiroyuki Browse this author →KAKEN DB | | Matsumoto, Misako Browse this author | | Seya, Tsukasa Browse this author |
| Keywords: | RIG-I | | virus | | type I interferon |
| Issue Date: | Sep-2011 |
| Publisher: | American Society for Microbiology |
| Journal Title: | Molecular and Cellular Biology |
| Volume: | 31 |
| Issue: | 18 |
| Start Page: | 3802 |
| End Page: | 3819 |
| Publisher DOI: | 10.1128/MCB.01368-10 |
| Abstract: | The cytoplasmic viral RNA sensors RIG-I and MDA5 are important for the production of type I interferon and other inflammatory cytokines. DDX60 is an uncharacterized DEXD/H box RNA helicase similar to Saccharomyces cerevisiae Ski2, a cofactor of RNA exosome, which is a protein complex required for the integrity of cytoplasmic RNA. Expression of DDX60 increases after viral infection, and the protein localizes at the cytoplasmic region. After viral infection, the DDX60 protein binds to endogenous RIG-I protein. The protein also binds to MDA5 and LGP2 but not to the downstream factors IPS-1 and I kappa B kinase epsilon (IKK-epsilon). Knockdown analysis shows that DDX60 is required for RIG-I-or MDA5-dependent type I interferon and interferon-inducible gene expression in response to viral infection. However, DDX60 is dispensable for TLR3-mediated signaling. Purified DDX60 helicase domains possess the activity to bind to viral RNA and DNA. Expression of DDX60 promotes the binding of RIG-I to double-stranded RNA. Taken together, our analyses indicate that DDX60 is a novel antiviral helicase promoting RIG-I-like receptor-mediated signaling. |
| Rights: | © 2011 American Society for Microbiology |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/48492 |
| Appears in Collections: | 医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
Submitter: 押海 裕之
|
