| Title: | Ciliates promote the transfer of the gene encoding the extended-spectrum -lactamase CTX-M-27 between Escherichia coli strains |
| Authors: | Oguri, Satoshi Browse this author |
| Matsuo, Junji Browse this author →KAKEN DB |
| Hayashi, Yasuhiro Browse this author →KAKEN DB |
| Nakamura, Shinji Browse this author →KAKEN DB |
| Hanawa, Tomoko Browse this author |
| Fukumoto, Tatsuya Browse this author |
| Mizutani, Yoshihiko Browse this author |
| Yao, Takashi Browse this author |
| Akizawa, Kouzi Browse this author |
| Suzuki, Haruki Browse this author |
| Shimizu, Chikara Browse this author →KAKEN DB |
| Matsuno, Kazuhiko Browse this author →KAKEN DB |
| Kamiya, Shigeru Browse this author |
| Yamaguchi, Hiroyuki Browse this author →KAKEN DB |
| Keywords: | ciliates |
| gene transfer |
| Escherichia coli |
| ESBL |
| CTX-M-27 |
| Issue Date: | Mar-2011 |
| Publisher: | The British Society for Antimicrobial Chemotherapy |
| Journal Title: | Journal of Antimicrobial Chemotherapy |
| Volume: | 66 |
| Issue: | 3 |
| Start Page: | 527 |
| End Page: | 530 |
| Publisher DOI: | 10.1093/jac/dkq487 |
| PMID: | 21177671 |
| Abstract: | OBJECTIVES:The mechanism by which Escherichia coli acquires multidrug resistance genes from other bacteria in the natural environment or livestock is still unclear. The ability of ciliates to promote the transfer of genes encoding extended-spectrum β-lactamases (ESBLs) between the CTX-M-27 donor and clinically isolated recipient E. coli strains was investigated.
METHODS:Equal amounts (∼10(9) cfu) of donor cefotaxime-resistant E. coli and recipient ciprofloxacin-resistant E. coli strains were mixed together in the presence or absence of 10(5) ciliates in Page's amoeba saline for 24 h, in the presence or absence of certain drugs (cytochalasin D, cycloheximide and latrunculin B).
RESULTS:Gene transfer frequency in the presence of ciliates was estimated at ∼10(-6); in the absence of ciliates it was ∼10(-10). Protein synthesis (cycloheximide) or phagocytosis (cytochalasin D or latrunculin B) inhibitors significantly reduced the frequency of gene transfer.
CONCLUSIONS:Ciliates promote the transfer of genes encoding ESBLs between E. coli strains, implying that the presence of ciliates may provide a significant impact on emerging multidrug-resistant bacteria. |
| Rights: | This is a pre-copy-editing, author-produced PDF of an article accepted for publication in Journal of Antimicrobial Chemotherapy following peer review. The definitive publisher-authenticated version is available online at: http://jac.oxfordjournals.org/content/66/3/527.full |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/48508 |
| Appears in Collections: | 保健科学院・保健科学研究院 (Graduate School of Health Sciences / Faculty of Health Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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