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Ciliates promote the transfer of the gene encoding the extended-spectrum  -lactamase CTX-M-27 between Escherichia coli strains

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Title: Ciliates promote the transfer of the gene encoding the extended-spectrum  -lactamase CTX-M-27 between Escherichia coli strains
Authors: Oguri, Satoshi Browse this author
Matsuo, Junji Browse this author →KAKEN DB
Hayashi, Yasuhiro Browse this author →KAKEN DB
Nakamura, Shinji Browse this author →KAKEN DB
Hanawa, Tomoko Browse this author
Fukumoto, Tatsuya Browse this author
Mizutani, Yoshihiko Browse this author
Yao, Takashi Browse this author
Akizawa, Kouzi Browse this author
Suzuki, Haruki Browse this author
Shimizu, Chikara Browse this author →KAKEN DB
Matsuno, Kazuhiko Browse this author →KAKEN DB
Kamiya, Shigeru Browse this author
Yamaguchi, Hiroyuki Browse this author →KAKEN DB
Keywords: ciliates
gene transfer
Escherichia coli
ESBL
CTX-M-27
Issue Date: Mar-2011
Publisher: The British Society for Antimicrobial Chemotherapy
Journal Title: Journal of Antimicrobial Chemotherapy
Volume: 66
Issue: 3
Start Page: 527
End Page: 530
Publisher DOI: 10.1093/jac/dkq487
PMID: 21177671
Abstract: OBJECTIVES:The mechanism by which Escherichia coli acquires multidrug resistance genes from other bacteria in the natural environment or livestock is still unclear. The ability of ciliates to promote the transfer of genes encoding extended-spectrum β-lactamases (ESBLs) between the CTX-M-27 donor and clinically isolated recipient E. coli strains was investigated. METHODS:Equal amounts (∼10(9) cfu) of donor cefotaxime-resistant E. coli and recipient ciprofloxacin-resistant E. coli strains were mixed together in the presence or absence of 10(5) ciliates in Page's amoeba saline for 24 h, in the presence or absence of certain drugs (cytochalasin D, cycloheximide and latrunculin B). RESULTS:Gene transfer frequency in the presence of ciliates was estimated at ∼10(-6); in the absence of ciliates it was ∼10(-10). Protein synthesis (cycloheximide) or phagocytosis (cytochalasin D or latrunculin B) inhibitors significantly reduced the frequency of gene transfer. CONCLUSIONS:Ciliates promote the transfer of genes encoding ESBLs between E. coli strains, implying that the presence of ciliates may provide a significant impact on emerging multidrug-resistant bacteria.
Rights: This is a pre-copy-editing, author-produced PDF of an article accepted for publication in Journal of Antimicrobial Chemotherapy following peer review. The definitive publisher-authenticated version is available online at: http://jac.oxfordjournals.org/content/66/3/527.full
Type: article (author version)
URI: http://hdl.handle.net/2115/48508
Appears in Collections:保健科学院・保健科学研究院 (Graduate School of Health Sciences / Faculty of Health Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 山口 博之

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