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Genotyping of sand fly species in Peruvian Andes where leishmaniasis is endemic
Title: | Genotyping of sand fly species in Peruvian Andes where leishmaniasis is endemic |
Authors: | Fujita, Megumi Browse this author | Kato, Hirotomo Browse this author →KAKEN DB | Cáceres, Abraham G. Browse this author | Gomez, Eduardo A. Browse this author | Velez, Lenin Browse this author | Mimori, Tatsuyuki Browse this author →KAKEN DB | Zhang, Feifei Browse this author | Iwata, Hiroyuki Browse this author →KAKEN DB | Korenaga, Masataka Browse this author | Sakurai, Tatsuya Browse this author | Katakura, Ken Browse this author →KAKEN DB | Hashiguchi, Yoshihisa Browse this author →KAKEN DB |
Keywords: | Genotyping | Sand fly | Lutzomyia | PCR-RFLP | 18S rRNA gene |
Issue Date: | Feb-2012 |
Publisher: | Elsevier B.V. |
Journal Title: | Acta Tropica |
Volume: | 121 |
Issue: | 2 |
Start Page: | 93 |
End Page: | 98 |
Publisher DOI: | 10.1016/j.actatropica.2011.10.004 |
Abstract: | Genotyping of sand fly species circulating in Peru was established on the basis of PCR-restriction fragment length polymorphisms (RFLPs) of the 18S ribosomal RNA (rRNA) gene. The sequences of 18S rRNA gene fragments from 12 Lutzomyia and 1 Warileya species were determined and their RFLP-patterns were analyzed. Consequently, RFLP analysis with the restriction enzyme AfaI and then HapII or KpnI, followed by XspI successfully differentiated them. Intraspecific genetic diversity affecting RFLP-patterns was not detected in the specimens collected from 24 areas of 8 departments. The genotyping was applied to the surveillance of sand flies collected from Andean areas where leishmaniasis is endemic, and its usability was verified. The present method promises to be a powerful tool for the classification and surveillance of sand flies circulating in Peru. |
Type: | article (author version) |
URI: | http://hdl.handle.net/2115/48545 |
Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 加藤 大智
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