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Genotyping of sand fly species in Peruvian Andes where leishmaniasis is endemic

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Title: Genotyping of sand fly species in Peruvian Andes where leishmaniasis is endemic
Authors: Fujita, Megumi Browse this author
Kato, Hirotomo Browse this author →KAKEN DB
Cáceres, Abraham G. Browse this author
Gomez, Eduardo A. Browse this author
Velez, Lenin Browse this author
Mimori, Tatsuyuki Browse this author →KAKEN DB
Zhang, Feifei Browse this author
Iwata, Hiroyuki Browse this author →KAKEN DB
Korenaga, Masataka Browse this author
Sakurai, Tatsuya Browse this author
Katakura, Ken Browse this author →KAKEN DB
Hashiguchi, Yoshihisa Browse this author →KAKEN DB
Keywords: Genotyping
Sand fly
Lutzomyia
PCR-RFLP
18S rRNA gene
Issue Date: Feb-2012
Publisher: Elsevier B.V.
Journal Title: Acta Tropica
Volume: 121
Issue: 2
Start Page: 93
End Page: 98
Publisher DOI: 10.1016/j.actatropica.2011.10.004
Abstract: Genotyping of sand fly species circulating in Peru was established on the basis of PCR-restriction fragment length polymorphisms (RFLPs) of the 18S ribosomal RNA (rRNA) gene. The sequences of 18S rRNA gene fragments from 12 Lutzomyia and 1 Warileya species were determined and their RFLP-patterns were analyzed. Consequently, RFLP analysis with the restriction enzyme AfaI and then HapII or KpnI, followed by XspI successfully differentiated them. Intraspecific genetic diversity affecting RFLP-patterns was not detected in the specimens collected from 24 areas of 8 departments. The genotyping was applied to the surveillance of sand flies collected from Andean areas where leishmaniasis is endemic, and its usability was verified. The present method promises to be a powerful tool for the classification and surveillance of sand flies circulating in Peru.
Type: article (author version)
URI: http://hdl.handle.net/2115/48545
Appears in Collections:獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 加藤 大智

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