White clover mosaic virus-induced gene silencing in pea


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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/48569

Title:	White clover mosaic virus-induced gene silencing in pea
Authors:	Ido, Yukari Browse this author
	Nakahara, Kenji S Browse this author →KAKEN DB
	Uyeda, Ichiro Browse this author →KAKEN DB
Keywords:	Pea
	RNA silencing
	Potexvirus
Issue Date:	2012
Publisher:	Springer
Journal Title:	Journal of General Plant Pathology
Volume:	78
Issue:	2
Start Page:	127
End Page:	132
Publisher DOI:	10.1007/s10327-012-0360-3
Abstract:	Double-stranded RNAs formed in secondary structures and replicative intermediates of viral genomes are thought to strongly elicit RNA silencing. This phenomenon is known as virus-induced gene silencing (VIGS). VIGS is a powerful tool for modifying gene expression in host plants. We constructed a virus vector based on White clover mosaic virus (WClMV) and demonstrated VIGS of phytoene desaturase (PDS) in pea. Photobleaching of tissues, caused by VIGS of PDS, was observed in restricted areas of upper leaves and stems. We confirmed that the PDS mRNA and subgenomic RNAs of WClMV were reduced in the photobleached tissues.
Type:	article (author version)
URI:	http://hdl.handle.net/2115/48569
Appears in Collections:	農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 中原健二

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