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White clover mosaic virus-induced gene silencing in pea

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Title: White clover mosaic virus-induced gene silencing in pea
Authors: Ido, Yukari Browse this author
Nakahara, Kenji S Browse this author →KAKEN DB
Uyeda, Ichiro Browse this author →KAKEN DB
Keywords: Pea
RNA silencing
Issue Date: 2012
Publisher: Springer
Journal Title: Journal of General Plant Pathology
Volume: 78
Issue: 2
Start Page: 127
End Page: 132
Publisher DOI: 10.1007/s10327-012-0360-3
Abstract: Double-stranded RNAs formed in secondary structures and replicative intermediates of viral genomes are thought to strongly elicit RNA silencing. This phenomenon is known as virus-induced gene silencing (VIGS). VIGS is a powerful tool for modifying gene expression in host plants. We constructed a virus vector based on White clover mosaic virus (WClMV) and demonstrated VIGS of phytoene desaturase (PDS) in pea. Photobleaching of tissues, caused by VIGS of PDS, was observed in restricted areas of upper leaves and stems. We confirmed that the PDS mRNA and subgenomic RNAs of WClMV were reduced in the photobleached tissues.
Type: article (author version)
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 中原 健二

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