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Modulation of Rat Cecal Microbiota by Administration of Raffinose and Encapsulated Bifidobacterium breve

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Title: Modulation of Rat Cecal Microbiota by Administration of Raffinose and Encapsulated Bifidobacterium breve
Authors: Dinoto, Achmad Browse this author
Suksomcheep, Akarat Browse this author
Ishizuka, Satoshi Browse this author →KAKEN DB
Kimura, Hanae Browse this author
Hanada, Satoshi Browse this author
Kamagata, Yoichi Browse this author
Asano, Kozo Browse this author →KAKEN DB
Tomita, Fusao Browse this author
Yokota, Atsushi9 Browse this author →KAKEN DB
Authors(alt): 横田, 篤9
Issue Date: Jan-2006
Publisher: American Society for Microbiology
Journal Title: Applied and Environmental Microbiology
Volume: 72
Issue: 1
Start Page: 784
End Page: 792
Publisher DOI: 10.1128/AEM.72.1.784-792.2006
Abstract: To investigate the effects of administration of raffinose and encapsulated Bifidobacterium breve JCM 1192T cells on the rat cecal microbiota, in a preclinical synbiotic study groups of male WKAH/Hkm Slc rats were fed for 3 weeks with four different test diets: basal diet (group BD), basal diet supplemented with raffinose (group RAF), basal diet supplemented with encapsulated B. breve (group CB), and basal diet supplemented with both raffinose and encapsulated B. breve (group RCB). The bacterial populations in cecal samples were determined by fluorescence in situ hybridization (FISH) and terminal restriction fragment length polymorphism (T-RFLP). B. breve cells were detected only in the RCB group and accounted for about 6.3% of the total cells as determined by FISH analysis. B. breve was also detected only in the RCB group by T-RFLP analysis. This was in contrast to the CB group, in which no B. breve signals were detected by either FISH or T-RFLP. Increases in the sizes of the populations of Bifidobacterium animalis, a Bifidobacterium indigenous to the rat, were observed in the RAF and RCB groups. Principal-component analysis of T-RFLP results revealed significant alterations in the bacterial populations of rats in the RAF and RCB groups; the population in the CB group was similar to that in the control group (group BD). To the best of our knowledge, these results provide the first clear picture of the changes in the rat cecal microbiota in response to synbiotic administration.
Rights: Copyright © 2006 American Society for Microbiology
Type: article (author version)
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 横田 篤

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