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北海道歯学雑誌 >
第32巻 第2号 >
Mechanism for propofol inhibition of Na+, K+-ATPase activity in rat brain
Title: | Mechanism for propofol inhibition of Na+, K+-ATPase activity in rat brain |
Authors: | Hase, Yuri Browse this author | Deyama, Yoshiaki Browse this author →KAKEN DB | Yoshimura, Yoshitaka Browse this author →KAKEN DB | Suzuki, Kuniaki Browse this author →KAKEN DB | Fukushima, Kazuaki Browse this author →KAKEN DB |
Keywords: | Intravenous anesthetic | propofol | Na+ | K+-ATPase |
Issue Date: | Mar-2012 |
Publisher: | 北海道歯学会 |
Journal Title: | 北海道歯学雑誌 |
Volume: | 32 |
Issue: | 2 |
Start Page: | 147 |
End Page: | 155 |
Abstract: | Propofol is one of the most widely used intravenous anesthetics, however the mechanism of the anesthetic effect is not fully understood. Na+,K+-ATPase is an enzyme present in all animal cell membranes and plays essential roles for the maintenance of neuronal excitability. There is a report of propofol inhibition of Na+,K+-ATPase activity, but the mechanism is not clearly established. To study the mechanism for propofol inhibition of Na+,K+-ATPase purified from whole brains of rats, the effects of propofol on Na+,K+-ATPase activity, Na+-ATPase, and K+-pNPPase activities, which are partial reactions of Na+,K+-ATPase were examined. Na+,K+-ATPase and Na+-ATPase activities decreased depending on the concentration of propofol, and were completely inhibited at 1.03 mM. Propofol decreased the maximum activity of Na+-, K+-, Mg2+-, and ATPdependent activation of Na+,K+-ATPase activity depending on its concentration, and changed the half maximal concentration for Na+, K+, and ATP, but not for Mg2+. Propofol also decreased the maximum activities of Na+-ATPase and K+-pNPPase, suggesting that propofol inhibits Na+,K+-ATPase activity by affecting the whole reaction process of Na+,K+-ATPase. The inhibition of Na+,K+-ATPase activity by propofol was reversible by dilution of its concentration. These results suggest that propofol reversibly inhibits Na+,K+-ATPase activity in a mixed-type inhibition pattern. |
Type: | article |
URI: | http://hdl.handle.net/2115/48709 |
Appears in Collections: | 北海道歯学雑誌 > 第32巻 第2号
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Submitter: 長谷 由理
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