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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Agriculture / Faculty of Agriculture >
Peer-reviewed Journal Articles, etc >
Polar localization and degradation of Arabidopsis boron transporters through distinct trafficking pathways
| Title: | Polar localization and degradation of Arabidopsis boron transporters through distinct trafficking pathways |
| Authors: | Takano, Junpei Browse this author | | Tanaka, Mayuki Browse this author | | Toyoda, Atsushi Browse this author | | Miwa, Kyoko Browse this author | | Kasai, Koji Browse this author | | Fuji, Kentaro Browse this author | | Onouchi, Hitoshi Browse this author | | Naito, Satoshi Browse this author →KAKEN DB | | Fujiwara, Toru Browse this author |
| Keywords: | plant nutrition | | radial transport | | homeostasis | | polarity | | endocytosis |
| Issue Date: | 16-Mar-2010 |
| Publisher: | National Academy of Sciences |
| Journal Title: | Proceedings of the National Academy of Sciences of the United States of America |
| Volume: | 107 |
| Issue: | 11 |
| Start Page: | 5220 |
| End Page: | 5225 |
| Publisher DOI: | 10.1073/pnas.0910744107 |
| Abstract: | Boron (B) is essential for plant growth, but is toxic when present in excess. In the roots of Arabidopsis thaliana under B limitation, a boric acid channel, NIP5;1, and a boric acid/borate exporter, BOR1, are required for efficient B uptake and subsequent translocation into the xylem, respectively. However, under high B conditions, BOR1 activity is repressed through endocytic degradation, presumably to avoid B toxicity. In this study, we investigated the localization of GFP-tagged NIP5;1 and BOR1 expressed under the control of their native promoters. Under B limitation, GFP-NIP5;1 and BOR1-GFP localized preferentially in outer (distal) and inner (proximal) plasma membrane (PM) domains, respectively, of various root cells. The polar localization of the boric acid channel and boric acid/borate exporter indicates the radial transport route of B toward the stele. Furthermore, mutational analysis revealed a requirement of tyrosine residues, in a probable cytoplasmic loop region of BOR1, for polar localization in various cells of the meristem and elongation zone. The same tyrosine residues were also required for vacuolar targeting upon high B supply. The present study of BOR1 and NIP5;1 demonstrates the importance of selective endocytic trafficking in polar localization and degradation of plant nutrient transporters for radial transport and homeostasis of plant mineral nutrients. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/49101 |
| Appears in Collections: | 農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 高野 順平
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