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Polar localization and degradation of Arabidopsis boron transporters through distinct trafficking pathways

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Title: Polar localization and degradation of Arabidopsis boron transporters through distinct trafficking pathways
Authors: Takano, Junpei Browse this author
Tanaka, Mayuki Browse this author
Toyoda, Atsushi Browse this author
Miwa, Kyoko Browse this author
Kasai, Koji Browse this author
Fuji, Kentaro Browse this author
Onouchi, Hitoshi Browse this author
Naito, Satoshi Browse this author →KAKEN DB
Fujiwara, Toru Browse this author
Keywords: plant nutrition
radial transport
homeostasis
polarity
endocytosis
Issue Date: 16-Mar-2010
Publisher: National Academy of Sciences
Journal Title: Proceedings of the National Academy of Sciences of the United States of America
Volume: 107
Issue: 11
Start Page: 5220
End Page: 5225
Publisher DOI: 10.1073/pnas.0910744107
Abstract: Boron (B) is essential for plant growth, but is toxic when present in excess. In the roots of Arabidopsis thaliana under B limitation, a boric acid channel, NIP5;1, and a boric acid/borate exporter, BOR1, are required for efficient B uptake and subsequent translocation into the xylem, respectively. However, under high B conditions, BOR1 activity is repressed through endocytic degradation, presumably to avoid B toxicity. In this study, we investigated the localization of GFP-tagged NIP5;1 and BOR1 expressed under the control of their native promoters. Under B limitation, GFP-NIP5;1 and BOR1-GFP localized preferentially in outer (distal) and inner (proximal) plasma membrane (PM) domains, respectively, of various root cells. The polar localization of the boric acid channel and boric acid/borate exporter indicates the radial transport route of B toward the stele. Furthermore, mutational analysis revealed a requirement of tyrosine residues, in a probable cytoplasmic loop region of BOR1, for polar localization in various cells of the meristem and elongation zone. The same tyrosine residues were also required for vacuolar targeting upon high B supply. The present study of BOR1 and NIP5;1 demonstrates the importance of selective endocytic trafficking in polar localization and degradation of plant nutrient transporters for radial transport and homeostasis of plant mineral nutrients.
Type: article (author version)
URI: http://hdl.handle.net/2115/49101
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 高野 順平

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