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Dental pulp can be a good candidate for nerve grafting in a xeno-graft model

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Title: Dental pulp can be a good candidate for nerve grafting in a xeno-graft model
Authors: Matsushita, Kazuhiro Browse this author →KAKEN DB
Wang, Wei Browse this author
Itoh, Soichiro Browse this author
Domon, Takanori Browse this author →KAKEN DB
Funahashi, Makoto Browse this author →KAKEN DB
Totsuka, Yasunori Browse this author →KAKEN DB
Keywords: Nerve grafting
Nerve regeneration
Dental pulp
Xenograft
Scaffold
Issue Date: 15-Apr-2012
Publisher: Elsevier B.V.
Journal Title: Journal of Neuroscience Methods
Volume: 205
Issue: 2
Start Page: 246
End Page: 251
Publisher DOI: 10.1016/j.jneumeth.2011.12.026
PMID: 22306062
Abstract: Dental pulp is discarded after extirpation of dental pulp and after tooth extraction. However, it contains nerve tissue abundantly and could be used more effectively. This study was designed to examine whether a dental pulp could be a candidate of donor for nerve grafting in xenografting model. The dental pulp was obtained from a human vital extracted tooth for orthodontic treatment, and treated with freezing and thawing method for reducing antigenicity. The treated sample was inserted into chitosan mesh tube for easy suturing, and then the complex was implanted into transected sciatic nerve in Sprague-Dawley (SD) rats (dental pulp group). As controls, chitosan tubes with and without sciatic nerve harvested from another SD rats were implanted (isograft group and tube group, respectively). As early as four weeks after grafting, regenerating axons accompanied by host Schwann cells were found to grout out through basal laminae by electron microscopy. The intact structure of basal laminae at this period suggested that they were derived from the original structure of donor graft. Twelve weeks after grafting, sporadic axonal regeneration was confirmed by light microscopy in the dental pulp group. Thirty-two weeks after implantation, aggregation of axons was observed in this group and matched that in isograft group. The average diameter of axons in dental pulp group was comparable to that in isograft group, whereas number of minifascicles and axon proportion were smaller. It was suggested that some delay occurred in dental pulp group because of the phagocytosis and absorption of tissue debris components remained after the freezing and thawing treatment. These findings clearly demonstrate that even dental pulp can act as conduits for regenerating axons.
Type: article (author version)
URI: http://hdl.handle.net/2115/49138
Appears in Collections:歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 松下 和裕

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