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Expression and localization of collagen type IV α1 chain in medaka ovary

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/49214

Title: Expression and localization of collagen type IV α1 chain in medaka ovary
Authors: Kato, Yumiko Browse this author
Ogiwara, Katsueki Browse this author →KAKEN DB
Fujimori, Chika Browse this author
Kimura, Atsushi Browse this author →KAKEN DB
Takahashi, Takayuki Browse this author →KAKEN DB
Keywords: medaka
ovary
collagen type IV α1
expression
distribution
Issue Date: Jun-2010
Publisher: Springer Berlin / Heidelberg
Journal Title: Cell and Tissue Research
Volume: 340
Issue: 3
Start Page: 595
End Page: 605
Publisher DOI: 10.1007/s00441-010-0969-5
PMID: 20424863
Abstract: A cDNA clone coding for collagen type IV α1 chain was obtained from the ovary of the medaka, Oryzias latipes. The clone encodes a protein of 1639 amino acids including a putative 21-residue signal peptide, and the deduced amino acid sequence of the α1 chain was homologous to those of the proteins from other species. Collagen type IV α1 chain mRNA was expressed in various tissues of the adult fish. In situ hybridization analysis revealed that the α1 chain mRNA was localized in the follicle layer of all growing follicles. In the post-ovulatory follicle that had released its oocyte in ovulation, the α1 chain transcript was detected in a winding line surrounding the tissue. This localization pattern was different from that of gelatinase B, a marker gene for granulosa cells. A specific antibody was prepared for the medaka collagen type IV α1 chain. Immunohistochemical analysis using this antibody yielded results consistent with those obtained by the in situ hybridization experiment. The current results indicate that in the medaka ovary, collagen type IV is synthesized by theca cells and is localized in the basement membrane.
Rights: The final publication is available at www.springerlink.com
Type: article (author version)
URI: http://hdl.handle.net/2115/49214
Appears in Collections:生命科学院・先端生命科学研究院 (Graduate School of Life Science / Faculty of Advanced Life Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 高橋 孝行

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