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Repair of Rabbit Osteochondral Defects by an Acellular Technique with an Ultrapurified Alginate Gel Containing Stromal Cell-Derived Factor-1

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Title: Repair of Rabbit Osteochondral Defects by an Acellular Technique with an Ultrapurified Alginate Gel Containing Stromal Cell-Derived Factor-1
Authors: Sukegawa, Atsushi Browse this author
Iwasaki, Norimasa Browse this author →KAKEN DB
Kasahara, Yasuhiko Browse this author
Onodera, Tomohiro Browse this author
Igarashi, Tatsuya Browse this author
Minami, Akio Browse this author →KAKEN DB
Issue Date: 1-May-2012
Publisher: Mary Ann Liebert
Journal Title: Tissue Engineering Part A
Volume: 18
Issue: 9-10
Start Page: 934
End Page: 945
Publisher DOI: 10.1089/ten.tea.2011.0380
PMID: 22097896
Abstract: The objective of this study was to determine whether the local administration of stromal cell-derived factor-1 (SDF-1) using ultrapurified alginate gel (UPAL gel) could improve reparative tissues of osteochondral defects compared with those without treatment. For the investigation, a full-thickness osteochondral defect 4.5mm in diameter and 3mm in depth was created in the patella groove of the distal femur in rabbits. Local expression of SDF-1 protein was temporarily upregulated at 1 week after creating the osteochondral defect. The local administration of SDF-1 enhanced the migration of host cells, mainly bone marrow stromal cells (BMSCs), to the site of the osteochondral defect. In vitro cell migration assay supported this result. In the SDF-1 (UPAL gel containing SDF-1) treatment group, the histological scores and the compressive modulus of reparative tissues were significantly improved compared with the no-treatment and vehicle (UPAL gel without SDF-1) groups. On the other hand, SDF-1 did not influence the cellular proliferation and chondrogenesis of BMSCs. Based on the results obtained here, we speculate that SDF-1 enhances the reparative process of osteochondral injuries not through direct effects on the behavior of host cells, but through increased migration of host cells to the injured site. UPAL gel, as a vehicle material, may play an important role in chondrogenesis of recruited cells, mainly BMSCs. The cell-free approach with local administration of SDF-1 may be an effective strategy for developing a minimally invasive technique for cartilage tissue regeneration.
Rights: This is a copy of an article published in the Tissue Engineering Part A © 2012 © Mary Ann Liebert, Inc.; Tissue Engineering Part A is available online at: http://www.liebertonline.com.
Type: article
URI: http://hdl.handle.net/2115/49281
Appears in Collections:医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 岩崎 倫政

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