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Virus-mediated efficient induction of epigenetic modifications of endogenous genes with phenotypic changes in plants

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Title: Virus-mediated efficient induction of epigenetic modifications of endogenous genes with phenotypic changes in plants
Authors: Kanazawa, Akira Browse this author →KAKEN DB
Inaba, Jun-ichi Browse this author
Shimura, Hanako Browse this author
Otagaki, Shungo Browse this author
Tsukahara, Sayuri Browse this author
Matsuzawa, Akihiko Browse this author
Kim, Bo Min Browse this author
Goto, Kazunori Browse this author
Masuta, Chikara Browse this author →KAKEN DB
Keywords: transcriptional gene silencing
epigenetic changes
RNA-directed DNA methylation
histone modification
Cucumber mosaic virus
short interfering RNA
Issue Date: Jan-2011
Publisher: Wiley-Blackwell
Journal Title: Plant Journal
Volume: 65
Issue: 1
Start Page: 156
End Page: 168
Publisher DOI: 10.1111/j.1365-313X.2010.04401.x
PMID: 21175898
Abstract: Gene silencing through transcriptional repression can be induced by targeting double-stranded RNA (dsRNA) to a gene promoter. It has been reported that a transgene was silenced by targeting dsRNA to the promoter, and the silenced state was inherited to the progeny plant even after removal of the silencing inducer from cells. In contrast, no plant has been produced that harbors silenced endogenous gene after removal of promoter-targeting dsRNA. Here, we show that heritable gene silencing can be induced by targeting dsRNA to the endogenous gene promoters in petunia and tomato plants, using the Cucumber mosaic virus (CMV)-based vector. We found that efficient silencing of endogenous genes depends on the function of the 2b protein encoded in the vector virus, which has the ability to facilitate epigenetic modifications through the transport of short interfering RNA to nucleus. Bisulfite sequencing analyses on the targeted promoter in the virus-infected and its progeny plants revealed that cytosine methylation was found not only at CG or CNG but also at CNN sites. The observed inheritance of asymmetric DNA methylation is quite unique, suggesting that plants have a mechanism to maintain even asymmetric methylation. This CMV-based gene silencing system provides a useful tool to artificially modify DNA methylation in plant genomes and elucidate the mechanism for epigenetic controls.
Rights: The definitive version is available at www.blackwell-synergy.com
Type: article (author version)
URI: http://hdl.handle.net/2115/49399
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 金澤 章

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