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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Agriculture / Faculty of Agriculture >
Peer-reviewed Journal Articles, etc >
Virus-mediated efficient induction of epigenetic modifications of endogenous genes with phenotypic changes in plants
| Title: | Virus-mediated efficient induction of epigenetic modifications of endogenous genes with phenotypic changes in plants |
| Authors: | Kanazawa, Akira Browse this author →KAKEN DB | | Inaba, Jun-ichi Browse this author | | Shimura, Hanako Browse this author | | Otagaki, Shungo Browse this author | | Tsukahara, Sayuri Browse this author | | Matsuzawa, Akihiko Browse this author | | Kim, Bo Min Browse this author | | Goto, Kazunori Browse this author | | Masuta, Chikara Browse this author →KAKEN DB |
| Keywords: | transcriptional gene silencing | | epigenetic changes | | RNA-directed DNA methylation | | histone modification | | Cucumber mosaic virus | | short interfering RNA |
| Issue Date: | Jan-2011 |
| Publisher: | Wiley-Blackwell |
| Journal Title: | Plant Journal |
| Volume: | 65 |
| Issue: | 1 |
| Start Page: | 156 |
| End Page: | 168 |
| Publisher DOI: | 10.1111/j.1365-313X.2010.04401.x |
| PMID: | 21175898 |
| Abstract: | Gene silencing through transcriptional repression can be induced by targeting double-stranded RNA (dsRNA) to a gene promoter. It has been reported that a transgene was silenced by targeting dsRNA to the promoter, and the silenced state was inherited to the progeny plant even after removal of the silencing inducer from cells. In contrast, no plant has been produced that harbors silenced endogenous gene after removal of promoter-targeting dsRNA. Here, we show that heritable gene silencing can be induced by targeting dsRNA to the endogenous gene promoters in petunia and tomato plants, using the Cucumber mosaic virus (CMV)-based vector. We found that efficient silencing of endogenous genes depends on the function of the 2b protein encoded in the vector virus, which has the ability to facilitate epigenetic modifications through the transport of short interfering RNA to nucleus. Bisulfite sequencing analyses on the targeted promoter in the virus-infected and its progeny plants revealed that cytosine methylation was found not only at CG or CNG but also at CNN sites. The observed inheritance of asymmetric DNA methylation is quite unique, suggesting that plants have a mechanism to maintain even asymmetric methylation. This CMV-based gene silencing system provides a useful tool to artificially modify DNA methylation in plant genomes and elucidate the mechanism for epigenetic controls. |
| Rights: | The definitive version is available at www.blackwell-synergy.com |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/49399 |
| Appears in Collections: | 農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 金澤 章
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