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Mechanism of increased respiration in an H+-ATPase-defective mutant of Corynebacterium glutamicum

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Title: Mechanism of increased respiration in an H+-ATPase-defective mutant of Corynebacterium glutamicum
Authors: Sawada, Kazunori Browse this author
Kato, Yui Browse this author
Imai, Keita Browse this author
Li, Liyuan Browse this author
Wada, Masaru Browse this author →KAKEN DB
Matsushita, Kazunobu Browse this author
Yokota, Atsushi Browse this author →KAKEN DB
Keywords: Corynebacterium glutamicum
H+-ATPase defect
Respiratory chain
NADH reoxidation
Energy metabolism
Energy deficiency
Issue Date: Apr-2012
Publisher: Society for Biotechnology, Japan
Journal Title: Journal of Bioscience and Bioengineering
Volume: 113
Issue: 4
Start Page: 467
End Page: 473
Publisher DOI: 10.1016/j.jbiosc.2011.11.021
PMID: 22188772
Abstract: We previously reported that a spontaneous H+-ATPase-defective mutant of Corynebacterium glutamicum, F172-8, derived from C. glutamicum ATCC 14067, showed enhanced glucose consumption and respiration rates. To investigate the genome-based mechanism of enhanced respiration rate in such C. glutamicum mutants, A-1, a H+-ATPase-defective mutant derived from C. glutamicum ATCC 13032, which harbors the same point mutation as F172-8, was used in this study. A-1 showed similar fermentation profiles to F172-8 when cultured in a jar fermentor. Enzyme activity measurements, quantitative real-time PCR, and DNA microarray analysis suggested that A-1 enhanced malate:quinone oxidoreductase/malate dehydrogenase and L-lactate dehydrogenase/NAD+-dependent-lactate dehydrogenase coupling reactions, but not NADH dehydrogenase-II, for reoxidation of the excess NADH arising from enhanced glucose consumption. A-1 also up-regulated succinate dehydrogenase, which may result in the relief of excess proton-motive force (pmf) in the H+-ATPase mutant. In addition, the transcriptional level of cytochrome bd oxidase, but not cytochrome bc1-aa3, also increased, which may help prevent the excess pmf generation caused by enhanced respiration. These results indicate that C. glutamicum possesses intriguing strategies for coping with NADH over-accumulation. Furthermore, these mechanisms are different from those in Escherichia coli, even though the two species use similar strategies to prevent excess pmf generation.
Type: article (author version)
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 澤田 和典

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