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Mechanism of increased respiration in an H+-ATPase-defective mutant of Corynebacterium glutamicum
Title: | Mechanism of increased respiration in an H+-ATPase-defective mutant of Corynebacterium glutamicum |
Authors: | Sawada, Kazunori Browse this author | Kato, Yui Browse this author | Imai, Keita Browse this author | Li, Liyuan Browse this author | Wada, Masaru Browse this author →KAKEN DB | Matsushita, Kazunobu Browse this author | Yokota, Atsushi Browse this author →KAKEN DB |
Keywords: | Corynebacterium glutamicum | H+-ATPase defect | Respiratory chain | NADH reoxidation | Energy metabolism | Energy deficiency |
Issue Date: | Apr-2012 |
Publisher: | Society for Biotechnology, Japan |
Journal Title: | Journal of Bioscience and Bioengineering |
Volume: | 113 |
Issue: | 4 |
Start Page: | 467 |
End Page: | 473 |
Publisher DOI: | 10.1016/j.jbiosc.2011.11.021 |
PMID: | 22188772 |
Abstract: | We previously reported that a spontaneous H+-ATPase-defective mutant of Corynebacterium glutamicum, F172-8, derived from C. glutamicum ATCC 14067, showed enhanced glucose consumption and respiration rates. To investigate the genome-based mechanism of enhanced respiration rate in such C. glutamicum mutants, A-1, a H+-ATPase-defective mutant derived from C. glutamicum ATCC 13032, which harbors the same point mutation as F172-8, was used in this study. A-1 showed similar fermentation profiles to F172-8 when cultured in a jar fermentor. Enzyme activity measurements, quantitative real-time PCR, and DNA microarray analysis suggested that A-1 enhanced malate:quinone oxidoreductase/malate dehydrogenase and L-lactate dehydrogenase/NAD+-dependent-lactate dehydrogenase coupling reactions, but not NADH dehydrogenase-II, for reoxidation of the excess NADH arising from enhanced glucose consumption. A-1 also up-regulated succinate dehydrogenase, which may result in the relief of excess proton-motive force (pmf) in the H+-ATPase mutant. In addition, the transcriptional level of cytochrome bd oxidase, but not cytochrome bc1-aa3, also increased, which may help prevent the excess pmf generation caused by enhanced respiration. These results indicate that C. glutamicum possesses intriguing strategies for coping with NADH over-accumulation. Furthermore, these mechanisms are different from those in Escherichia coli, even though the two species use similar strategies to prevent excess pmf generation. |
Type: | article (author version) |
URI: | http://hdl.handle.net/2115/49426 |
Appears in Collections: | 農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 澤田 和典
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