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A novel virus-like double-stranded RNA in an obligate biotroph arbuscular mycorrhizal fungus: a hidden player in mycorrhizal symbiosis

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Title: A novel virus-like double-stranded RNA in an obligate biotroph arbuscular mycorrhizal fungus: a hidden player in mycorrhizal symbiosis
Authors: Ikeda, Yoji Browse this author
Shimura, Hanako Browse this author
Kitahara, Ryoko Browse this author
Masuta, Chikara Browse this author
Ezawa, Tatsuhiro Browse this author →KAKEN DB
Issue Date: 2012
Publisher: APS Press
Journal Title: Molecular Plant-Microbe Interactions
Volume: 25
Issue: 7
Start Page: 120314052115002
Publisher DOI: 10.1094/MPMI-11-11-0288
PMID: 22414436
Abstract: Arbuscular mycorrhizal (AM) fungi form mutualistic associations with most land plants and enhance phosphorus uptake of the host plants. Fungal viruses (mycoviruses) that possess a double-stranded RNA (dsRNA) genome often impact on plant-fungal interactions via altering phenotypic expression of their host fungi. The present study for the first time demonstrates the presence of dsRNAs, which is highly likely to be mycoviruses, in AM fungi. dsRNA was extracted from mycelia of Glomus sp. RF1, purified, and subjected to electrophoresis. The fungus was found to harbor various dsRNA segments that differed in size. Among them, a 4.5-kbp segment was termed Glomus sp. RF1 virus-like-medium dsRNA (GRF1V-M) and characterized in detail. The GRF1V-M genome segment was 4,557 nucleotides in length and encoded RNA-dependent RNA polymerase and a structural protein. GRF1V-M was phylogenetically distinct and could not be assigned to known genera of mycovirus. The GRF1V-M-free culture line of Glomus sp. RF1, which was raised by single spore isolation, produced two-fold greater number of spores and promoted plant growth more efficiently than the GRF1V-M-positive lines. These observations suggest that mycoviruses in AM fungi, at least some of them, have evolved under unique selection pressures and are a biologically active component in the symbiosis.
Type: article
URI: http://hdl.handle.net/2115/49461
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 江澤 辰広

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