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Tumor-Derived Microvesicles Induce Proangiogenic Phenotype in Endothelial Cells via Endocytosis
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| Title: | Tumor-Derived Microvesicles Induce Proangiogenic Phenotype in Endothelial Cells via Endocytosis |
| Authors: | Kawamoto, Taisuke Browse this author | | Ohga, Noritaka Browse this author →KAKEN DB | | Akiyama, Kosuke Browse this author | | Hirata, Naoya Browse this author | | Kitahara, Shuji Browse this author | | Maishi, Nako Browse this author | | Osawa, Takahiro Browse this author | | Yamamoto, Kazuyuki Browse this author | | Kondoh, Miyako Browse this author | | Shindoh, Masanobu Browse this author →KAKEN DB | | Hida, Yasuhiro Browse this author →KAKEN DB | | Hida, Kyoko Browse this author →KAKEN DB |
| Issue Date: | 30-Mar-2012 |
| Publisher: | Public Library of Science |
| Journal Title: | PLoS One |
| Volume: | 7 |
| Issue: | 3 |
| Start Page: | e34045 |
| Publisher DOI: | 10.1371/journal.pone.0034045 |
| Abstract: | Background: Increasing evidence indicates that tumor endothelial cells (TEC) differ from normal endothelial cells (NEC). Our previous reports also showed that TEC were different from NEC. For example, TEC have chromosomal abnormality and proangiogenic properties such as high motility and proliferative activity. However, the mechanism by which TEC acquire a specific character remains unclear. To investigate this mechanism, we focused on tumor-derived microvesicles (TMV). Recent studies have shown that TMV contain numerous types of bioactive molecules and affect normal stromal cells in the tumor microenvironment. However, most of the functional mechanisms of TMV remain unclear. Methodology/Principal Findings: Here we showed that TMV isolated from tumor cells were taken up by NEC through endocytosis. In addition, we found that TMV promoted random motility and tube formation through the activation of the phosphoinositide 3-kinase/Akt pathway in NEC. Moreover, the effects induced by TMV were inhibited by the endocytosis inhibitor dynasore. Our results indicate that TMV could confer proangiogenic properties to NEC partly via endocytosis. Conclusion: We for the first time showed that endocytosis of TMV contributes to tumor angiogenesis. These findings offer new insights into cancer therapies and the crosstalk between tumor and endothelial cells mediated by TMV in the tumor microenvironment. |
| Rights: | http://creativecommons.org/licenses/by/3.0/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/49570 |
| Appears in Collections: | 歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 樋田 京子
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