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Ciliates Expel Environmental Legionella-laden Pellets for Stockpiling Food

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/49712

Title: Ciliates Expel Environmental Legionella-laden Pellets for Stockpiling Food
Authors: Hojo, Fuhito Browse this author
Sato, Daisuke Browse this author
Matsuo, Junji Browse this author →KAKEN DB
Miyake, Masaki Browse this author
Nakamura, Shinji Browse this author →KAKEN DB
Kunichika, Miyuki Browse this author
Hayashi, Yasuhiro Browse this author →KAKEN DB
Yoshida, Mitsutaka Browse this author
Takahashi, Kaori Browse this author
Takemura, Hiromu Browse this author
Kamiya, Shigeru Browse this author
Yamaguchi, Hiroyuki Browse this author →KAKEN DB
Issue Date: Aug-2012
Publisher: American Society for Microbiology
Journal Title: Applied and Environmental Microbiology
Volume: 78
Issue: 15
Start Page: 5247
End Page: 5257
Publisher DOI: 10.1128/AEM.00421-12
Abstract: When the ciliate Tetrahymena is cultured with Legionella pneumophila, they expel bacteria packaged in free spherical pellets. Why the ciliates expel these pellets remains unclear. Hence, we determined optimal conditions for pellet expulsion, and assessed whether they contribute to maintenance of growth and survival of ciliates. When incubated environmental L. pneumophila, the ciliates maximally expelled the pellets at 2 days after infection. Heat-killed bacteria failed to produce pellets from ciliates, and there was no obvious difference in pellet production among the ciliates or bacterial strains. Morphological studies with assessment of lipid accumulation showed that pellets contained tightly packed bacteria with rapid lipid accumulation and were composed of the layers of membranes; bacterial culturability in the pellets rapidly decreased in contrast to that in ciliate-free culture, although the bacteria maintained membrane integrity in the pellets. Furthermore, ciliates newly cultured with pellets were maintained and grew vigorously compared with those without pellets. In contrast, a human L. pneumophila isolate killed ciliates 7 day post-infection in a Dot/Icm dependent manner and pellets harboring this strain did not support ciliate growth. Also, pellets harboring the human isolate were resuscitated by co-culture with amoebae, depending on Dot/Icm expression. Thus, while ciliates expel pellet-packaged environmental L. pneumophila for stockpiling food, the pellets packaged the human isolate are harmful on ciliate's survival, possibly connecting clinical significance.
Type: article (author version)
URI: http://hdl.handle.net/2115/49712
Appears in Collections:保健科学院・保健科学研究院 (Graduate School of Health Sciences / Faculty of Health Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 山口 博之

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