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Prediction of maturational competence of feline oocytes using supravital staining of cumulus cells by propidium iodide

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Title: Prediction of maturational competence of feline oocytes using supravital staining of cumulus cells by propidium iodide
Authors: Uchikura, Kenzo Browse this author
Nagano, Masashi Browse this author →KAKEN DB
Hishinuma, Mitsugu Browse this author
Keywords: Cat
Follicle
IVM
Membrane integrity
Propidium iodide
Issue Date: May-2011
Publisher: Cambridge University Press
Journal Title: Zygote
Start Page: 1
End Page: 5
Publisher DOI: 10.1017/S096719941100027X
Abstract: We examined the relationship between integrityof Cumulus cells and nuclear maturation rate after in vitro culture to determine a non-invasive prediction of the maturationalcompetence of feline oocytes・ Feline cumulus-oocyte complexes (COCs) were collected from either small (400-800l九m) or large (≧800 l⊥m) follicles・ Immediately after collection′ cumulus cells were evaluated morphologically (thickness of cumulus cell layers) and stained with propidium iodide (PI)′ which penetrates only nonviable cells・ Cumulus cells without PI staining were judged as having good membrane integrity・ After evaluation, COCs were cultured for 30 h and their nuclear maturation rate was determined. The nuclear maturation rate of oocytes derived from large follicles (89・8%) was higher (p < 0・05) than that from small follicles (60・8%)・ There was no difference in the maturation rate of oocytes from follicleswith the same size regardless of cumulus morphology ln contrast′ Oocytes that had cumulus cells with good membrane integrity showed a higher mahration rate (93・8%) than oocytes with poor cumulus integrity (76・9%) in large follicles (p < 0・05)・ We conclude that evaluation of membrane integrity of cumulus cells by propidium iodide stainlng Can be used to predict the maturational competence of oocytes・
Rights: Cambridge University Press
Relation: http://journals.cambridge.org/action/displayAbstract?fromPage=online&aid=8277023
Type: article
URI: http://hdl.handle.net/2115/49780
Appears in Collections:獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 永野 昌志

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