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Effects of molecular structural variants on serum Krebs von den Lungen-6 levels in sarcoidosis

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Title: Effects of molecular structural variants on serum Krebs von den Lungen-6 levels in sarcoidosis
Authors: Shigemura, Masahiko Browse this author
Nasuhara, Yasuyuki Browse this author →KAKEN DB
Konno, Satoshi Browse this author →KAKEN DB
Shimizu, Chikara Browse this author →KAKEN DB
Matsuno, Kazuhiko Browse this author →KAKEN DB
Yamguchi, Etsuro Browse this author
Nishimura, Masaharu Browse this author →KAKEN DB
Keywords: Serum KL-6
Molecular structural variant
Sarcoidosis
Issue Date: 31-May-2012
Publisher: BioMed Central
Journal Title: Journal of Translational Medicine
Volume: 10
Start Page: 111
Publisher DOI: 10.1186/1479-5876-10-111
Abstract: Background: Serum Krebs von den Lungen-6 (KL-6), which is classified as human mucin-1 (MUC1), is used as a marker of sarcoidosis and other interstitial lung diseases. However, there remain some limitations due to a lack of information on the factors contributing to increased levels of serum KL-6. This study was designed to investigate the factors contributing to increased levels of serum KL-6 by molecular analysis. Methods: Western blot analysis using anti-KL-6 antibody was performed simultaneously on the bronchoalveolar lavage fluid (BALF) and serum obtained from 128 subjects with sarcoidosis. Results: KL-6/MUC1 in BALF showed three bands and five band patterns. These band patterns were associated with the MUC1 genotype and the KL-6 levels. KL-6/MUC1 band patterns in serum were dependent on molecular size class in BALF. Significantly increased levels of serum KL-6, serum/BALF KL-6 ratio and serum soluble interleukin 2 receptor were observed in the subjects with influx of high molecular size KL-6/MUC1 from the alveoli to blood circulation. The multivariate linear regression analysis involving potentially relevant variables such as age, gender, smoking status, lung parenchymal involvement based on radiographical stage and molecular size of KL-6/MUC1 in serum showed that the molecular size of KL-6/MUC1 in serum was significant independent determinant of serum KL-6 levels. Conclusions: The molecular structural variants of KL-6/MUC1 and its leakage behavior affect serum levels of KL-6 in sarcoidosis. This information may assist in the interpretation of serum KL-6 levels in sarcoidosis.
Rights: http://creativecommons.org/licenses/by/2.0
Type: article
URI: http://hdl.handle.net/2115/49840
Appears in Collections:北海道大学病院 (Hokkaido University Hospital) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 南須原 康行

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