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Distribution of putative denitrifying methane oxidizing bacteria in sediment of a freshwater lake, Lake Biwa

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Title: Distribution of putative denitrifying methane oxidizing bacteria in sediment of a freshwater lake, Lake Biwa
Authors: Kojima, Hisaya Browse this author →KAKEN DB
Tsutsumi, Masazumi Browse this author
Ishikawa, Kanako Browse this author
Iwata, Tomoya Browse this author
Mußmann, Marc Browse this author
Fukui, Manabu Browse this author →KAKEN DB
Keywords: Methane oxidation
Freshwater lake
NC10 bacteria
Issue Date: Jun-2012
Publisher: Elsevier
Journal Title: Systematic and Applied Microbiology
Volume: 35
Issue: 4
Start Page: 233
End Page: 238
Publisher DOI: 10.1016/j.syapm.2012.03.005
PMID: 22504019
Abstract: Methane oxidation coupled to denitrification is mediated by 'Candidatus methylomirabilis oxyfera', which belongs to the candidate phylum NC10. The distribution of putative denitrifying methane oxidizing bacteria related to "M. oxyfera" was investigated in a freshwater lake, Lake Biwa, Japan. In the surface layer of the sediment from a profundal site, a phylotype closely related to "M. oxyfera" was most frequently detected among NC10 bacteria in PCR analysis of the 16S rRNA gene. In the sediment, sequences related to "M. oxyfera" were also detected in a pmoA gene library. The presence of NC10 bacteria was also confirmed by catalyzed reporter deposition fluorescence in situ hybridization. Denaturing gradient gel electrophoresis (DGGE) and quantitative real-time PCR indicated that the abundance of "M. oxyfera"-related phylotype was higher in the upper layers of the profundal sediment. The horizontal distribution of the putative methanotrophs in sediment of the lake was also analyzed by DGGE, which revealed that their occurrence was restricted to deep water areas. These results agreed with those in a previous study of another freshwater lake, and suggested that the upper layer of the profundal sediments is major the habitat for denitrifying methanotrophs.
Type: article (author version)
Appears in Collections:低温科学研究所 (Institute of Low Temperature Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 小島 久弥

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