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The Peptide Nucleic Acid-Locked Nucleic Acid Polymerase Chain Reaction Clamp-Based Test for Epidermal Growth Factor Receptor Mutations in Bronchoscopic Cytological Specimens of Non-Small Cell Lung Cancer

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Title: The Peptide Nucleic Acid-Locked Nucleic Acid Polymerase Chain Reaction Clamp-Based Test for Epidermal Growth Factor Receptor Mutations in Bronchoscopic Cytological Specimens of Non-Small Cell Lung Cancer
Authors: Yamada, Noriyuki Browse this author
Oizumi, Satoshi Browse this author →KAKEN DB
Asahina, Hajime Browse this author
Shinagawa, Naofumi Browse this author
Kikuchi, Eiki Browse this author →KAKEN DB
Kikuchi, Junko Browse this author
Sakakibara-Konishi, Jun Browse this author →KAKEN DB
Tanaka, Tomoaki Browse this author
Kobayashi, Kunihiko Browse this author
Hagiwara, Koichi Browse this author
Nishimura, Masaharu Browse this author →KAKEN DB
Keywords: Non-small cell lung cancer
Epidermal growth factor receptor mutations
Bronchoscopy
Cytological specimens
EGFR mutations
Issue Date: Jul-2012
Publisher: Karger
Journal Title: Oncology
Volume: 82
Issue: 6
Start Page: 341
End Page: 346
Publisher DOI: 10.1159/000338327
PMID: 22677909
Abstract: Objectives: Cytological examination of samples obtained by bronchoscopy is a useful method for establishing the diagnosis of non-small cell lung cancer (NSCLC). However, the utility of a highly sensitive method for the detection of epidermal growth factor receptor (EGFR) mutation in the cytological specimens has not been fully evaluated. Methods: We retrospectively examined the efficacy of the peptide nucleic acid-locked nucleic acid polymerase chain reaction clamp (PNA-LNA PCR clamp) method for detecting EGFR mutations in 122 bronchoscopic cytological specimens from NSCLC patients. Results: Overall, 41 specimens (33.6%) were positive for EGFR mutation. Twenty-nine (39.7%) of 73 specimens obtained by using endobronchial ultrasonography with a guide sheath, 7 (33.3%) of 21 specimens obtained under direct vision by using a conventional bronchoscope, 4 (36.4%) of 11 specimens obtained by using an ultrathin bronchoscope, and 1 (5.9%) of 17 specimens obtained by endobronchial ultrasound-guided transbronchial needle aspiration were positive for EGFR mutation. Furthermore, among 22 resected NSCLC cases, the EGFR mutation status obtained from bronchoscopic materials was consistent with the status obtained from surgical samples, with the exception of one case. Conclusion: The detection of EGFR mutation by subjecting bronchoscopic cytological specimens to a PNA-LNA PCR clamp assay proves useful.
Rights: © 2012 S. Karger AG, Basel
Type: article (author version)
URI: http://hdl.handle.net/2115/50057
Appears in Collections:北海道大学病院 (Hokkaido University Hospital) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 大泉 聡史

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