| Title: | The Peptide Nucleic Acid-Locked Nucleic Acid Polymerase Chain Reaction Clamp-Based Test for Epidermal Growth Factor Receptor Mutations in Bronchoscopic Cytological Specimens of Non-Small Cell Lung Cancer |
| Authors: | Yamada, Noriyuki Browse this author |
| Oizumi, Satoshi Browse this author →KAKEN DB |
| Asahina, Hajime Browse this author |
| Shinagawa, Naofumi Browse this author |
| Kikuchi, Eiki Browse this author →KAKEN DB |
| Kikuchi, Junko Browse this author |
| Sakakibara-Konishi, Jun Browse this author →KAKEN DB |
| Tanaka, Tomoaki Browse this author |
| Kobayashi, Kunihiko Browse this author |
| Hagiwara, Koichi Browse this author |
| Nishimura, Masaharu Browse this author →KAKEN DB |
| Keywords: | Non-small cell lung cancer |
| Epidermal growth factor receptor mutations |
| Bronchoscopy |
| Cytological specimens |
| EGFR mutations |
| Issue Date: | Jul-2012 |
| Publisher: | Karger |
| Journal Title: | Oncology |
| Volume: | 82 |
| Issue: | 6 |
| Start Page: | 341 |
| End Page: | 346 |
| Publisher DOI: | 10.1159/000338327 |
| PMID: | 22677909 |
| Abstract: | Objectives: Cytological examination of samples obtained by bronchoscopy is a useful method for establishing the diagnosis of non-small cell lung cancer (NSCLC). However, the utility of a highly sensitive method for the detection of epidermal growth factor receptor (EGFR) mutation in the cytological specimens has not been fully evaluated. Methods: We retrospectively examined the efficacy of the peptide nucleic acid-locked nucleic acid polymerase chain reaction clamp (PNA-LNA PCR clamp) method for detecting EGFR mutations in 122 bronchoscopic cytological specimens from NSCLC patients. Results: Overall, 41 specimens (33.6%) were positive for EGFR mutation. Twenty-nine (39.7%) of 73 specimens obtained by using endobronchial ultrasonography with a guide sheath, 7 (33.3%) of 21 specimens obtained under direct vision by using a conventional bronchoscope, 4 (36.4%) of 11 specimens obtained by using an ultrathin bronchoscope, and 1 (5.9%) of 17 specimens obtained by endobronchial ultrasound-guided transbronchial needle aspiration were positive for EGFR mutation. Furthermore, among 22 resected NSCLC cases, the EGFR mutation status obtained from bronchoscopic materials was consistent with the status obtained from surgical samples, with the exception of one case. Conclusion: The detection of EGFR mutation by subjecting bronchoscopic cytological specimens to a PNA-LNA PCR clamp assay proves useful. |
| Rights: | © 2012 S. Karger AG, Basel |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/50057 |
| Appears in Collections: | 北海道大学病院 (Hokkaido University Hospital) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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