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β-Glucan Derived from Aureobasidium pullulans Is Effective for the Prevention of Influenza in Mice

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Title: β-Glucan Derived from Aureobasidium pullulans Is Effective for the Prevention of Influenza in Mice
Authors: Muramatsu, Daisuke Browse this author
Iwai, Atsushi Browse this author
Aoki, Shiho Browse this author
Uchiyama, Hirohumi Browse this author
Kawata, Koji Browse this author
Nakayama, Yosuke Browse this author
Nikawa, Yasuhiro Browse this author
Kusano, Kisato Browse this author
Okabe, Mitsuyasu Browse this author
Miyazaki, Tadaaki Browse this author →KAKEN DB
Issue Date: 23-Jul-2012
Publisher: Public Library of Science
Journal Title: PLoS One
Volume: 7
Issue: 7
Start Page: e41399
Publisher DOI: 10.1371/journal.pone.0041399
Abstract: β-(1→3)-D-glucans with β-(1→6)-glycosidic linked branches produced by mushrooms, yeast and fungi are known to be an immune activation agent, and are used in anti-cancer drugs or health-promoting foods. In this report, we demonstrate that oral administration of Aureobasidium pullulans-cultured fluid (AP-CF) enriched with the β-(1→3),(1→6)-D-glucan exhibits efficacy to protect mice infected with a lethal titer of the A/Puerto Rico/8/34 (PR8; H1N1) strain of influenza virus. The survival rate of the mice significantly increased by AP-CF administration after sublethal infection of PR8 virus. The virus titer in the mouse lung homogenates was significantly decreased by AP-CF administration. No significant difference in the mRNA expression of inflammatory cytokines, and in the population of lymphocytes was observed in the lungs of mice administered with AP-CF. Interestingly, expression level for the mRNA of virus sensors, RIG-I (retinoic acid-inducible gene-I) and MDA5 (melanoma differentiation-associated protein 5) strongly increased at 5 hours after the stimulation of A. pullulans-produced purified β-(1→3),(1→6)-D-glucan (AP-BG) in murine macrophage-derived RAW264.7 cells. Furthermore, the replication of PR8 virus was significantly repressed by pre-treatment of AP-BG. These findings suggest the increased expression of virus sensors is effective for the prevention of influenza by the inhibition of viral replication with the administration of AP-CF.
Rights: http://creativecommons.org/licenses/by/3.0/
Type: article
URI: http://hdl.handle.net/2115/50063
Appears in Collections:遺伝子病制御研究所 (Institute for Genetic Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 宮崎 忠昭

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