|
Hokkaido University Collection of Scholarly and Academic Papers >
Institute for Genetic Medicine >
Peer-reviewed Journal Articles, etc >
β-Glucan Derived from Aureobasidium pullulans Is Effective for the Prevention of Influenza in Mice
This item is licensed under:Creative Commons Attribution 3.0 Unported
| Title: | β-Glucan Derived from Aureobasidium pullulans Is Effective for the Prevention of Influenza in Mice |
| Authors: | Muramatsu, Daisuke Browse this author | | Iwai, Atsushi Browse this author | | Aoki, Shiho Browse this author | | Uchiyama, Hirohumi Browse this author | | Kawata, Koji Browse this author | | Nakayama, Yosuke Browse this author | | Nikawa, Yasuhiro Browse this author | | Kusano, Kisato Browse this author | | Okabe, Mitsuyasu Browse this author | | Miyazaki, Tadaaki Browse this author →KAKEN DB |
| Issue Date: | 23-Jul-2012 |
| Publisher: | Public Library of Science |
| Journal Title: | PLoS One |
| Volume: | 7 |
| Issue: | 7 |
| Start Page: | e41399 |
| Publisher DOI: | 10.1371/journal.pone.0041399 |
| Abstract: | β-(1→3)-D-glucans with β-(1→6)-glycosidic linked branches produced by mushrooms, yeast and fungi are known to be an immune activation agent, and are used in anti-cancer drugs or health-promoting foods. In this report, we demonstrate that oral administration of Aureobasidium pullulans-cultured fluid (AP-CF) enriched with the β-(1→3),(1→6)-D-glucan exhibits efficacy to protect mice infected with a lethal titer of the A/Puerto Rico/8/34 (PR8; H1N1) strain of influenza virus. The survival rate of the mice significantly increased by AP-CF administration after sublethal infection of PR8 virus. The virus titer in the mouse lung homogenates was significantly decreased by AP-CF administration. No significant difference in the mRNA expression of inflammatory cytokines, and in the population of lymphocytes was observed in the lungs of mice administered with AP-CF. Interestingly, expression level for the mRNA of virus sensors, RIG-I (retinoic acid-inducible gene-I) and MDA5 (melanoma differentiation-associated protein 5) strongly increased at 5 hours after the stimulation of A. pullulans-produced purified β-(1→3),(1→6)-D-glucan (AP-BG) in murine macrophage-derived RAW264.7 cells. Furthermore, the replication of PR8 virus was significantly repressed by pre-treatment of AP-BG. These findings suggest the increased expression of virus sensors is effective for the prevention of influenza by the inhibition of viral replication with the administration of AP-CF. |
| Rights: | http://creativecommons.org/licenses/by/3.0/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/50063 |
| Appears in Collections: | 遺伝子病制御研究所 (Institute for Genetic Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
Submitter: 宮崎 忠昭
|
