HUSCAP logo Hokkaido Univ. logo

Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine >
Japanese Journal of Veterinary Research >
Volume 60, Number 2&3 >

Inhibitory effects of an M2-specific monoclonal antibody on different strains of influenza A virus

Files in This Item:
JJVR60-2-3_003.pdf1.8 MBPDFView/Open
Please use this identifier to cite or link to this item:

Title: Inhibitory effects of an M2-specific monoclonal antibody on different strains of influenza A virus
Authors: Muto, Nilton Akio Browse this author
Yoshida, Reiko Browse this author
Suzuki, Tadaki Browse this author →KAKEN DB
Kobayashi, Shintaro Browse this author
Ozaki, Hiroichi Browse this author
Fujikura, Daisuke Browse this author
Manzoor, Rashid Browse this author
Muramatsu, Mieko Browse this author
Takada, Ayato Browse this author →KAKEN DB
Kimura, Takashi Browse this author →KAKEN DB
Sawa, Hirofumi Browse this author →KAKEN DB
Keywords: HA
influenza A virus
inhibitory activity
M2e antibody
Issue Date: Aug-2012
Publisher: Graduate School of Veterinary Medicine, Hokkaido University
Journal Title: Japanese Journal of Veterinary Research
Volume: 60
Issue: 2&3
Start Page: 71
End Page: 83
Abstract: New approaches to the treatment of influenza have been designed based on the highly conserved antigenicity of the M2 envelope protein among influenza A virus strains. The present study examined the anti-viral activities of an anti-M2 ectodomain (M2e) monoclonal antibody (clone rM2ss23), which binds to the M2 proteins of the influenza A virus strains A/Aichi/2/68 (H3N2) (Aichi) and A/PR/8/34 (H1N1) (PR8). The results showed that rM2ss23 bound to both Aichi and PR8 M2 proteins expressed on the cell surface. While the antibody did not prevent virus entry into cells, it significantly inhibited plaque formation by the Aichi strain in a dose-dependent manner when infected cells were cultured in the presence of the antibody. By contrast, the growth of PR8 (H1N1) was not affected by the antibody. A reverse genetics approach revealed that the inhibitory effect of rM2ss23 on the Aichi virus was abolished by replacing the genes encoding the HA and/or M proteins with those of the PR8 strain. These results suggest that rM2ss23 prevents virus release from infected cells and further suggest that the mechanisms underlying the virus budding mediates by HA and M2 proteins might differ between the Aichi and PR8 strains.
Type: bulletin (article)
Appears in Collections:Japanese Journal of Veterinary Research > Volume 60, Number 2&3

Submitter: 獣医学部図書室

Export metadata:

OAI-PMH ( junii2 , jpcoar )

MathJax is now OFF:


Feedback - Hokkaido University