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Development and evaluation of indirect enzyme-linked immunosorbent assay for a screening test to detect antibodies against classical swine fever virus

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Please use this identifier to cite or link to this item:http://doi.org/10.14943/jjvr.60.2-3.85

Title: Development and evaluation of indirect enzyme-linked immunosorbent assay for a screening test to detect antibodies against classical swine fever virus
Authors: Sakoda, Yoshihiro Browse this author →KAKEN DB
Wakamoto, Hiroaki Browse this author
Tamura, Tehpin Browse this author
Nomura, Takushi Browse this author
Naito, Michiko Browse this author
Aoki, Hiroshi Browse this author
Morita, Hiroshi Browse this author
Kida, Hiroshi Browse this author →KAKEN DB
Fukusho, Akio Browse this author
Keywords: antibody
classical swine fever
ELISA
pestivirus
Issue Date: Aug-2012
Publisher: Graduate School of Veterinary Medicine, Hokkaido University
Journal Title: Japanese Journal of Veterinary Research
Volume: 60
Issue: 2&3
Start Page: 85
End Page: 94
Abstract: An indirect enzyme-linked immunosorbent assay (ELISA) was developed for a screening test to detect antibodies against classical swine fever virus (CSFV). Viral glycoproteins, which were purified from swine kidney cells infected with CSFV ALD/A76 strain by the immunoaffinity purification using monoclonal antibody against E2 protein, were adsorbed on a microtiter plate as the antigen for the antibody detection. Each antibody titer of serum sample was expressed as a sample per positive value calculated with optical absorbance of each sample and that of a positive control. The advantage of this ELISA is its higher sensitivity: most sera containing more than 4 neutralization titers were determined to be positive. This ELISA is unable to discriminate between antibodies against CSFV and those against other ruminant pestiviruses, therefore positive sera in this ELISA should be evaluated by a cross-neutralization test using CSFV, bovine viral diarrhea virus, and border disease virus. Taken together, the indirect ELISA developed in this study is useful screening tool to detect antibodies against CSFV for the large-scale monitoring of classical swine fever.
Type: bulletin (article)
URI: http://hdl.handle.net/2115/50097
Appears in Collections:Japanese Journal of Veterinary Research > Volume 60, Number 2&3

Submitter: 獣医学部図書室

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