|
Hokkaido University Collection of Scholarly and Academic Papers >
Theses >
博士 （理学） >
Studies on the Om(IE) locus of Drosophila ananassae
| Title: | Studies on the Om(IE) locus of Drosophila ananassae |
| Authors: | Juni, Naoto1 Browse this author |
| Authors(alt): | 従二, 直人1 |
| Issue Date: | 24-Mar-1995 |
| Publisher: | Hokkaido University |
| Abstract: | Optic morphology (Om) mutations in Drosophila ananassae are a group of retrotransposon (tom)-induced gain-of-function mutations which map to at least 22 independent loci and exclusively affect the compound eye morphology. Unlike all the other Om mutants which are characterized by fewer-than-normal and disorganized ommatidia， the Om(1E) mutants exceptionally possess enlarged compound eyes， giving impression that one extra eye is fused dorso-posteriorly to another． In order to characterize the Om(1E) mutation and the Om(1E) gene，I have carried out molecular analyses． A putative Om(1E) locus cloned by tom-tagging and chromosome walking contained two transcribed regions in the vicinity of tom insertion sites of the Om(1E) mutant alleles， and one of these regions was shown to be the Om(1E) gene by analyses of gene expression patterns in wild-type and the mutants， and by production of the phenocopy by P-element-mediated transformation with Drosophila melanogaster． Sequence analysis showed that the Om(1E) gene encodes a novel protein having potential transmembrane domain(s)．in situ hybridization and immunocytochemical analyses demonstrated that the Om(1E) gene is expressed ubiquitously in embryonic cells， imaginal discs， and the central nervous system of third instar larvae， and specifically in lamina precursor cells． Artificial ubiquitous over－expression of Om(1E) or its antisense RNA affected morphogenesis of wing imaginal disc derivatives and/or large bristle formation． These findings suggest that the Om(1E) gene is involved in various biological events． |
| Conffering University: | 北海道大学 |
| Degree Report Number: | 甲第3533号 |
| Degree Level: | 博士 |
| Degree Discipline: | 理学 |
| Type: | theses (doctoral) |
| URI: | http://hdl.handle.net/2115/50139 |
| Appears in Collections: | 学位論文 (Theses) > 博士 （理学）
|
Submitter: 従二 直人
|
