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Identification and expression analysis of the genes involved in serotonin biosynthesis and transduction in the field cricket Gryllus bimaculatus
| Title: | Identification and expression analysis of the genes involved in serotonin biosynthesis and transduction in the field cricket Gryllus bimaculatus |
| Authors: | Watanabe, Takayuki Browse this author | | Sadamoto, Hisayo Browse this author | | Aonuma, Hitoshi Browse this author →KAKEN DB |
| Keywords: | serotonin | | tryptophan hydroxylase | | phenylalanine-tryptophan hydroxylase | | aromatic L-amino acid decarboxylase | | serotonin receptors | | Gryllus bimaculatus |
| Issue Date: | Oct-2011 |
| Publisher: | Wiley-Blackwell |
| Journal Title: | Insect Molecular Biology |
| Volume: | 20 |
| Issue: | 5 |
| Start Page: | 619 |
| End Page: | 635 |
| Publisher DOI: | 10.1111/j.1365-2583.2011.01093.x |
| PMID: | 21699597 |
| Abstract: | Serotonin (5-HT) modulates various aspects of behaviours such as aggressive behaviour and circadian behaviour in the cricket. To elucidate the molecular basis of the cricket 5-HT system, we identified 5-HT-related genes in the field cricket Gryllus bimaculatus DeGeer. Complementary DNA of tryptophan hydroxylase (TRH) and phenylalanine-tryptophan hydroxylase (TPH) which convert tryptophan into 5-hydroxy-L-tryptophan (5-HTP), and that of aromatic L-amino acid decarboxylase (AADC) which converts 5-HTP into 5-HT were isolated from a cricket brain cDNA library. In addition, four 5-HT receptor genes (5-HT_[1A], 5-HT_[1B], 5-HT_[2α], and 5-HT7) were identified. Expression analysis of TRH and TPH, which are selectively involved in neuronal and peripheral 5-HT synthesis in Drosophila, suggested that two 5-HT synthesis pathways co-exist in the cricket neuronal tissues. The four of 5-HT receptor genes were expressed in various tissues at differential expression levels, suggesting that the 5-HT system is widely distributed in the cricket. |
| Rights: | The definitive version is available at http://onlinelibrary.wiley.com/ |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/50147 |
| Appears in Collections: | 電子科学研究所 (Research Institute for Electronic Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 青沼 仁志
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