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Purification and biochemical characterization of a cellulase from the digestive organs of the short-spined sea urchin Strongylocentrotus intermedius

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Title: Purification and biochemical characterization of a cellulase from the digestive organs of the short-spined sea urchin Strongylocentrotus intermedius
Authors: Hasegawa, Syuto Browse this author
Ura, Kazuhiro Browse this author →KAKEN DB
Tanaka, Hiroyuki Browse this author →KAKEN DB
Ojima, Takao Browse this author →KAKEN DB
Takagi, Yasuaki Browse this author →KAKEN DB
Keywords: Cellulase
Digestive organ
Enzymatic properties
Protein isolation
Sea urchin
Issue Date: Sep-2012
Publisher: Springer Japan
Journal Title: Fisheries Science
Volume: 78
Issue: 5
Start Page: 1107
End Page: 1115
Publisher DOI: 10.1007/s12562-012-0528-y
Abstract: We isolated a cellulase from the digestive organs of the short-spined sea urchin Strogylocentrotus intermedius using a combination of ion-exchange chromatography and gel filtration together with an assay for carboxymethylcellulase activity. The isolated cellulase was stained as a single band by Congo red. The molecular weight of the isolated cellulase, as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis under reducing conditions, was 59 kDa. The isolated cellulase exhibited hydrolytic activity toward carboxymethyl cellulose, with an optimum temperature and pH of 30℃ and pH 8.0, respectively. The thermal stability of the enzyme was characterized by determining the temperature at which activity decreased by 50 % with treatment for 30 min at pH 7.0 and found to be 32℃. Cellulase activity remained at a high level at 5-20℃, which is the growth temperature of the short-spined sea urchin. These results confirm that the short-spined sea urchin should preferably be reared at a water temperature of < 20℃.
Rights: © 2012 公益社団法人日本水産学会
© 2012 The Japanese Society of Fisheries Science
Type: article
URI: http://hdl.handle.net/2115/50293
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 浦 和寛

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