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Purification and biochemical characterization of a cellulase from the digestive organs of the short-spined sea urchin Strongylocentrotus intermedius
Title: | Purification and biochemical characterization of a cellulase from the digestive organs of the short-spined sea urchin Strongylocentrotus intermedius |
Authors: | Hasegawa, Syuto Browse this author | Ura, Kazuhiro Browse this author →KAKEN DB | Tanaka, Hiroyuki Browse this author →KAKEN DB | Ojima, Takao Browse this author →KAKEN DB | Takagi, Yasuaki Browse this author →KAKEN DB |
Keywords: | Cellulase | Digestive organ | Enzymatic properties | Protein isolation | Sea urchin |
Issue Date: | Sep-2012 |
Publisher: | Springer Japan |
Journal Title: | Fisheries Science |
Volume: | 78 |
Issue: | 5 |
Start Page: | 1107 |
End Page: | 1115 |
Publisher DOI: | 10.1007/s12562-012-0528-y |
Abstract: | We isolated a cellulase from the digestive organs of the short-spined sea urchin Strogylocentrotus intermedius using a combination of ion-exchange chromatography and gel filtration together with an assay for carboxymethylcellulase activity. The isolated cellulase was stained as a single band by Congo red. The molecular weight of the isolated cellulase, as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis under reducing conditions, was 59 kDa. The isolated cellulase exhibited hydrolytic activity toward carboxymethyl cellulose, with an optimum temperature and pH of 30℃ and pH 8.0, respectively. The thermal stability of the enzyme was characterized by determining the temperature at which activity decreased by 50 % with treatment for 30 min at pH 7.0 and found to be 32℃. Cellulase activity remained at a high level at 5-20℃, which is the growth temperature of the short-spined sea urchin. These results confirm that the short-spined sea urchin should preferably be reared at a water temperature of < 20℃. |
Rights: | © 2012 公益社団法人日本水産学会 | © 2012 The Japanese Society of Fisheries Science |
Type: | article |
URI: | http://hdl.handle.net/2115/50293 |
Appears in Collections: | 水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 浦 和寛
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