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Construction of a replicon and an infectious cDNA clone of the Sofjin strain of the Far-Eastern subtype of tick-borne encephalitis virus

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Title: Construction of a replicon and an infectious cDNA clone of the Sofjin strain of the Far-Eastern subtype of tick-borne encephalitis virus
Authors: Takano, Ayako Browse this author
Yoshii, Kentaro Browse this author →KAKEN DB
Omori-Urabe, Yuki Browse this author
Yokozawa, Kana Browse this author
Kariwa, Hiroaki Browse this author →KAKEN DB
Takashima, Ikuo Browse this author →KAKEN DB
Issue Date: Nov-2011
Publisher: Springer Vienna
Journal Title: Archives of Virology
Volume: 156
Issue: 11
Start Page: 1931
End Page: 1941
Publisher DOI: 10.1007/s00705-011-1066-0
PMID: 21785855
Abstract: Tick-borne encephalitis virus (TBEV) causes severe encephalitis in humans. The Sofjin-HO strain is the prototype strain of the TBEV Far-Eastern subtype and is highly pathogenic in a mouse model. In this study, we constructed replicons and infectious cDNA clones of the Sofjin-HO strain. The replication of the replicon RNA was confirmed, and infectious viruses were recovered from the infectious cDNA clone. The recombinant viruses showed similar virulence characteristics to those of the parental virus. While characterizing the replicon and infectious cDNA, several amino acid differences derived from cell culture adaptations were analysed. The amino acids differences at E position 496 and NS4A position 58 were found to affect viral replication. The Gly- or Ala-to-Glu substitution at E position 122 was shown to increase neuroinvasiveness in mice. These replicons and infectious cDNA clones are useful in revealing the viral molecular determinant involved in the replication and pathogenicity of TBEV.
Rights: The original publication is available at www.springerlink.com
Type: article (author version)
URI: http://hdl.handle.net/2115/50373
Appears in Collections:獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 好井 健太朗

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