HUSCAP logo Hokkaido Univ. logo

Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Environmental Science / Faculty of Environmental Earth Science >
Peer-reviewed Journal Articles, etc >

Changes in activity and transcript level of liver and gill metabolic enzymes during smoltification in wild and hatchery-reared masu salmon (Oncorhynchus masou)

Files in This Item:
Aqu362-363_109-120.pdf491.49 kBPDFView/Open
Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/50686

Title: Changes in activity and transcript level of liver and gill metabolic enzymes during smoltification in wild and hatchery-reared masu salmon (Oncorhynchus masou)
Authors: Mizuno, Shinya Browse this author
Urabe, Hirokazu Browse this author
Aoyama, Tomoya Browse this author
Omori, Hajime Browse this author
Iijima, Anai Browse this author
Kasugai, Kiyoshi Browse this author
Torao, Mitsuru Browse this author
Misaka, Naoyuki Browse this author
Koide, Nobuhisa Browse this author
Ueda, Hiroshi Browse this author →KAKEN DB
Keywords: Metabolic enzyme
Smoltification
Masu salmon
Liver
Gill
Issue Date: 28-Sep-2012
Publisher: Elsevier B.V.
Journal Title: Aquaculture
Volume: 362-363
Start Page: 109
End Page: 120
Publisher DOI: 10.1016/j.aquaculture.2010.10.034
Abstract: It is important for success of the masu salmon, Oncorhynchus masou, stock enhancement program in Hokkaido (northern Japan) to demonstrate physiological problems in hatchery-reared (hatchery) smolt for artificial release. The present study examined changes in liver and gill metabolic parameters in wild and hatchery masu salmon during smoltification and elucidated differences in hepatic and gill metabolism between wild and hatchery fish. As reference to freshwater-adapted wild and hatchery smolt in this study, metabolic parameters of coastal smolt were studied. Yearling wild and hatchery smolting fish were collected from the Ken-ichi River and the Donan Research Branch, which used Ken-ichi river water for fish culture, at the same time every month from March through May, 2008. Coastal smolts were caught from Nemuro Bay of Hokkaido in June. Decreased hepatic glycogen content during smoltification, which was observed in wild fish and revealed activation of glycogenolysis, was not found in hatchery fish. Hatchery fish demonstrated a positive change in hepatic ATP content during smoltification, while wild fish showed negative change in the content, which reflected activated consumption of hepatic ATP stores during smoltification. Increases in gill pyruvate kinase activity during smoltification, which were found in wild fish and indicated activation of glycolysis, were not detected in hatchery fish. There was a difference in increased timing of hepatic citrate synthase activity during smoltification between hatchery and wild fish. Increased gill citrate synthase activity during smoltification, which was observed in wild fish and reflected enhancement of the citric acid cycle, was not found in hatchery fish. Hatchery smolt revealed lower liver cytochrome c oxidase activity and transcript levels of some respiratory chain enzymes compared to wild smolt in May, which suggested lower respiratory chain capacity in hatchery fish at mid-smolt stage. On the other hand, there were no remarkable differences in hepatic and gill 3-hydroxyacyl-coenzyme A dehydrogenase related to lipolysis and creatine kinase activities, which operate in resolution of creatine phosphate, during smoltification between hatchery and wild fish. These results suggested hatchery masu salmon had some metabolic problems with carbohydrate metabolism, the citric acid cycle, and the respiratory chain. Our study will give valuable information to improve physiological quality of hatchery smolt for artificial release.
Type: article (author version)
URI: http://hdl.handle.net/2115/50686
Appears in Collections:環境科学院・地球環境科学研究院 (Graduate School of Environmental Science / Faculty of Environmental Earth Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 上田 宏

Export metadata:

OAI-PMH ( junii2 , jpcoar )

MathJax is now OFF:


 

Feedback - Hokkaido University