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Molecular Characterization of Multidrug-Resistant Mycobacterium tuberculosis Isolated in Nepal

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Title: Molecular Characterization of Multidrug-Resistant Mycobacterium tuberculosis Isolated in Nepal
Authors: Poudel, Ajay Browse this author
Nakajima, Chie Browse this author →KAKEN DB
Fukushima, Yukari Browse this author
Suzuki, Haruka Browse this author
Pandey, Basu Dev Browse this author
Maharjan, Bhagwan Browse this author
Suzuki, Yasuhiko Browse this author →KAKEN DB
Keywords: Multi drug-resistant
Mycobacterium tuberculosis
rpoB
katG
inhA
Issue Date: Jun-2012
Publisher: American Society for Microbiology
Journal Title: Antimicrobial Agents and Chemotherapy
Volume: 56
Issue: 6
Start Page: 2831
End Page: 2836
Publisher DOI: 10.1128/AAC.06418-11
Abstract: Despite being one of the first countries globally to introduce multidrug-resistant tuberculosis (MDR-TB) case management, the number of MDR-TB cases is continuing to rise in Nepal. Rapid molecular tests applicable in this setting to identify resistant organisms would be an effective tool in reversing this trend. To develop such tools, information about the frequency and distribution of mutations that are associated with phenotypic drug resistance in Mycobacterium tuberculosis is required. In the present study, we investigated the prevalence of mutations in rpoB and katG genes and the inhA promoter region in 158 M. tuberculosis isolates, 109 phenotypically MDR and 49 non-MDR collected in Nepal, by DNA sequencing. Mutations affecting the 81-bp rifampicin (RIF) resistance-determining region (RRDR) of rpoB were identified in 106 of 109 (97.3%) RIF-resistant isolates. The codons most frequently affected were 531, 526 and 516 with percentages of 58.7%, 15.6% and 15.6%, respectively. Of 113 isoniazid (INH)-resistant isolates, 99 (87.6%) had mutations in the katG gene, with Ser315Thr being the most prevalent (81.4%) substitution. Mutations in the inhA promoter region were detected in 14 (12.4%) INH-resistant isolates. The results from this study provide an overview of the current situation of RIF and INH resistance in M. tuberculosis in Nepal and can serve as a basis for developing or improving rapid molecular-based tests to monitor drug-resistant strains in this country.
Rights: © 2012 American Society for Microbiology
Type: article (author version)
URI: http://hdl.handle.net/2115/50780
Appears in Collections:人獣共通感染症リサーチセンター (Research Center for Zoonosis Control) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 鈴木 定彦

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