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Novel monoclonal antibody recognizing triglyceride-rich oxidized LDLs associated with severe liver disease and small oxidized LDLs in normal subjects

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Title: Novel monoclonal antibody recognizing triglyceride-rich oxidized LDLs associated with severe liver disease and small oxidized LDLs in normal subjects
Authors: Sakurai, Toshihiro Browse this author
Ichikawa, Ayako Browse this author
Furukawa, Hiroyuki Browse this author
Wada, Norio Browse this author
Nagasaka, Atsushi Browse this author
Takahashi, Yuji Browse this author
Fujikawa, Masato Browse this author
Ikuta, Akiko Browse this author
Furumaki, Hiroaki Browse this author
Shiga, Maiko Browse this author
Shimizu, Chikara Browse this author →KAKEN DB
Hui, Shu-Ping Browse this author →KAKEN DB
Jin, Shigeki Browse this author
Takeda, Seiji Browse this author →KAKEN DB
Fuda, Hirotoshi Browse this author
Nagasaka, Hironori Browse this author
Kobayashi, Seiichi Browse this author →KAKEN DB
Chiba, Hitoshi Browse this author →KAKEN DB
Issue Date: Sep-2012
Publisher: Royal Society of Medicine Press
Journal Title: Annals of Clinical Biochemistry
Volume: 49
Issue: 5
Start Page: 456
End Page: 462
Publisher DOI: 10.1258/acb.2012.011284
PMID: 22807504
Abstract: Background: Triglyceride-rich low-density lipoproteins (TG-rich LDLs) in the plasma of patients with severe liver disease are reported to change macrophages into foam cells in vitro. Methods: Male BALB/c mice were immunized with TG-rich LDLs isolated from the plasma of a patient with severe liver disease. The resulting monoclonal antibody (G11-6) was used in a sandwich enzyme-linked immunosorbent assay (ELISA) in combination with polyclonal anti-apolipoprotein B antibodies. The time course of copper-mediated LDL oxidation was monitored using this ELISA. The results were compared to those of the two commercial ELISAs for oxidized LDL using DLH or ML25, thiobarbituric acid reactive substances (TBARS), and the optical absorbance for the conjugated dienes generated in lipid peroxides. Further, the lipoprotein fractions separated by gel filtration were tested with this ELISA in healthy volunteers (n = 11) and patients (n = 3) with liver disease. Results: G11-6 reacted with oxidized LDLs during only the early phase of copper-oxidation, being distinct from the other monoclonal antibodies and methods. G11-6 was confirmed to react with TG-rich LDLs in patients, while it reacted with small LDL particles in normal controls. Conclusions: The monoclonal antibody G11-6 is useful for detecting oxidized small LDLs in normal controls and oxidized TG-rich LDLs in patients with severe liver disease.
Rights: Ann Clin Biochem, September 2012, vol. 49 no. 5, pp.456-462, doi: 10.1258/acb.2012.011284. This is the final draft, after peer-review, of a manuscript published in Annals of Clinical Biochemistry. The definitive version, detailed above, is available online at
Type: article (author version)
Appears in Collections:保健科学院・保健科学研究院 (Graduate School of Health Sciences / Faculty of Health Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 櫻井 俊宏

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