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Protection against white spot syndrome virus (WSSV) infection in kuruma shrimp orally vaccinated with WSSV rVP26 and rVP28
Title: | Protection against white spot syndrome virus (WSSV) infection in kuruma shrimp orally vaccinated with WSSV rVP26 and rVP28 |
Authors: | Satoh, Jun Browse this author | Nishizawa, Toyohiko Browse this author →KAKEN DB | Yoshimizu, Mamoru Browse this author →KAKEN DB |
Keywords: | Oral vaccination | White spot syndrome virus | WSSV | Marsupenaeus japonicus | Kuruma shrimp | Quasi-immune response |
Issue Date: | 20-Nov-2008 |
Publisher: | Inter-Research |
Journal Title: | Diseases of Aquatic Organisms |
Volume: | 82 |
Issue: | 2 |
Start Page: | 89 |
End Page: | 96 |
Publisher DOI: | 10.3354/dao01978 |
Abstract: | White spot syndrome virus (WSSV) is the causative agent of white spot disease (WSD), one of the most serious diseases affecting global shrimp farming. We compared WSSV infection induction in kuruma shrimp Marsupenaeus japonicus by oral, immersion, and intramuscular injection (IM) exposure methods and evaluated the oral vaccine prepared from the recombinant WSSV proteins rVP26 and rVP28. The 50% lethal doses (LD50) of WSSV by oral, immersion, and IM challenges were 10^[-0.4], 10^[-4.4], and 10^[-7.7] g shrimp^[-1], respectively, indicating that WSSV infection efficiency by oral challenge was significantly less than the other 2 challenge routes. However, in shrimp farms it is believed that WSSV infection is easily and commonly established by the oral route as a result of cannibalization of WSSV-infected shrimp. Kuruma shrimp vaccinated orally with WSSV rVP26 or rVP28 were challenged with WSSV by oral, immersion, and IM routes to compare protection efficacy. The relative percent survival values were 100% for oral challenge, 70 to 71% for immersion, and 34 to 61% for IM. Thus, the protection against WSSV-infection that was induced in kuruma shrimp by oral vaccination with rVP26 or rVP28 seemed equivalent to that obtained through IM vaccination. |
Rights: | © 2008 Inter-Research |
Type: | article |
URI: | http://hdl.handle.net/2115/50889 |
Appears in Collections: | 水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 吉水 守
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