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Comparative genomic analysis of the proteasome β5t subunit gene : implications for the origin and evolution of thymoproteasomes

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Title: Comparative genomic analysis of the proteasome β5t subunit gene : implications for the origin and evolution of thymoproteasomes
Authors: Sutoh, Yoichi Browse this author
Kondo, Mizuho Browse this author
Ohta, Yuko Browse this author
Ota, Tatsuya Browse this author
Tomaru, Utano Browse this author →KAKEN DB
Flajnik, Martin F. Browse this author
Kasahara, Masanori Browse this author →KAKEN DB
Keywords: Thymoproteasome
β5t subunit
Issue Date: Jan-2012
Publisher: Springer-Verlag
Journal Title: Immunogenetics
Volume: 64
Issue: 1
Start Page: 49
End Page: 58
Publisher DOI: 10.1007/s00251-011-0558-0
Abstract: The thymoproteasome is a recently discovered, specialized form of 20S proteasomes expressed exclusively in the thymic cortex. Although the precise molecular mechanism by which the thymoproteasome exerts its function remains to be elucidated, accumulating evidence indicates that it plays a crucial role in positive selection of T cells. In the present study, we analyzed the evolution of the β5t subunit, a β-type catalytic subunit uniquely present in thymoproteasomes. The gene coding for the β5t subunit, designated PSMB11, was identified in the cartilaginous fish, the most divergent group of jawed vertebrates compared to the other jawed vertebrates, but not in jawless vertebrates or invertebrates. Interestingly, teleost fish have two copies of apparently functional PSMB11 genes, designated PSMB11a and PSMB11b, that encode β5t subunits with distinct amino acids in the S1 pocket. BLAST searches of genome databases suggest that birds such as chickens, turkey, and zebra finch lost the PSMB11 gene, and have neither thymoproteasomes nor immunoproteasomes. In mammals, reptiles, amphibians, and teleost fishes, the PSMB11 gene (the PSMB11a gene in teleost fish) is located next to the PSMB5 gene coding for the β5 subunit of the standard 20S proteasome, indicating that the PSMB11 gene arose by tandem duplication from the evolutionarily more ancient PSMB5 gene. The general absence of introns in PSMB11 and an unusual exon-intron structure of jawed vertebrate PSMB5 suggest that PSMB5 lost introns and duplicated in tandem in a common ancestor of jawed vertebrates, with PSMB5 subsequently gaining two introns and PSMB11 remaining intronless.
Rights: The original publication is available at
Type: article (author version)
Appears in Collections:医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 笠原 正典

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