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Elicitation of Both Anti HIV-1 Env Humoral and Cellular Immunities by Replicating Vaccinia Prime Sendai Virus Boost Regimen and Boosting by CD40Lm

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Title: Elicitation of Both Anti HIV-1 Env Humoral and Cellular Immunities by Replicating Vaccinia Prime Sendai Virus Boost Regimen and Boosting by CD40Lm
Authors: Zhang, Xianfeng Browse this author →KAKEN DB
Sobue, Tomoyoshi Browse this author
Isshiki, Mao Browse this author
Makino, Shun-ichi Browse this author
Inoue, Makoto Browse this author
Kato, Kazunori Browse this author
Shioda, Tatsuo Browse this author
Ohashi, Takashi Browse this author →KAKEN DB
Sato, Hirotaka Browse this author
Komano, Jun Browse this author
Hanabusa, Hideji Browse this author
Shida, Hisatoshi Browse this author →KAKEN DB
Issue Date: 7-Dec-2012
Publisher: Public Library of Science
Journal Title: PLoS One
Volume: 7
Issue: 12
Start Page: e51633
Publisher DOI: 10.1371/journal.pone.0051633
Abstract: For protection from HIV-1 infection, a vaccine should elicit both humoral and cell-mediated immune responses. A novel vaccine regimen and adjuvant that induce high levels of HIV-1 Env-specific T cell and antibody (Ab) responses was developed in this study. The prime-boost regimen that used combinations of replication-competent vaccinia LC16m8Δ(m8Δ) and Sendai virus (SeV) vectors expressing HIV-1 Env efficiently produced both Env-specific CD8+ T cells and anti-Env antibodies, including neutralizing antibodies (nAbs). These results sharply contrast with vaccine regimens that prime with an Env expressing plasmid and boost with the m8Δ or SeV vector that mainly elicited cellular immunities. Moreover, co-priming with combinations of m8Δs expressing Env or a membrane-bound human CD40 ligand mutant (CD40Lm) enhanced Env-specific CD8+ T cell production, but not anti-Env antibody production. In contrast, priming with an m8Δ that coexpresses CD40Lm and Env elicited more anti-Env Abs with higher avidity, but did not promote T cell responses. These results suggest that the m8Δ prime/SeV boost regimen in conjunction with CD40Lm expression could be used as an immunization platform for driving both potent cellular and humoral immunities against pathogens such as HIV-1.
Type: article
Appears in Collections:遺伝子病制御研究所 (Institute for Genetic Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 志田 壽利

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