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Study of transcriptional regulation of rRNA genes in higher palnts
| Title: | Study of transcriptional regulation of rRNA genes in higher palnts |
| Other Titles: | 高等植物におけるrRNA遺伝子の転写調節機構に関する研究 |
| Authors: | Suzuki, Akihiro1 Browse this author |
| Authors(alt): | 鈴木, 章弘1 |
| Issue Date: | 25-Dec-1995 |
| Publisher: | Hokkaido University |
| Abstract: | I have been studying the mechanisms of transcription of rRNA in higher plants. Here, 1 present the complete nucleotide sequence of IGS region of Daucus carota rDNA. The repeatmg unit of Daucus carota rDNA has two major size classes and this polymorphism was caused by reiteration of a 460 bp sequence. Further analysis in this paper shows the possibility that Daucus carota rDNA has plural promoter sequences. The results of S1 analysis, and gel-retardation analysis show the possibility that one of the sequences may act as a spacer promoter, like Xenopus, Drosophila and Arabidopsis. In order to identify the promoter sequences, I used Vicia faba rDNA as a material. From the analysis using in vitro transcription system, I showed the possibility that the cis element required for transcription of Vicia faba rDNA may locate in downstream sequence from -363. This region contains the sequence which binds to the nuclear protein fraction with sequence-specific manner. I expanded this analysis of DNA-protein interaction. I fractionated the Vicia faba nuclear extracts using ion-exchange chromatography and affinity chromatography, and showed that the binding protein fraction divided to three kinds of protein fractions. They recognized similar sequences for binding, but their binding stability was different. Furthermore, I was able to isolate a cDNA clone encoded DNA binding protein that interact with the sequence near the transcription start site of rRNA gene by southwestern technique. From these results, I discussed the general mechanisms of transcription of plant rRNA gene. |
| Conffering University: | 北海道大学 |
| Degree Report Number: | 甲第3722号 |
| Degree Level: | 博士 |
| Degree Discipline: | 理学 |
| Type: | theses (doctoral) |
| URI: | http://hdl.handle.net/2115/51292 |
| Appears in Collections: | 学位論文 (Theses) > 博士 （理学）
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Submitter: 鈴木 章弘
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