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Glutamate transporter GLAST is expressed in the radial glia-astrocyte lineage of developing mouse spinal cord.

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Title: Glutamate transporter GLAST is expressed in the radial glia-astrocyte lineage of developing mouse spinal cord.
Authors: Shibata, T. Browse this author
Yamada, K. Browse this author
Watanabe, M. Browse this author
Ikenaka, K. Browse this author
Wada, K. Browse this author
Tanaka, K. Browse this author
Inoue, Y. Browse this author
Issue Date: 1-Dec-1997
Publisher: Society for Neuroscience
Journal Title: The Journal of neuroscience : the official journal of the Society for Neuroscience
Volume: 17
Issue: 23
Start Page: 9212
End Page: 9219
Publisher DOI: 10.1523/JNEUROSCI.17-23-09212.1997
PMID: 09364068
Abstract: The glutamate transporter GLAST is localized on the cell membrane of mature astrocytes and is also expressed in the ventricular zone of developing brains. To characterize and follow the GLAST-expressing cells during development, we examined the mouse spinal cord by in situ hybridization and immunohistochemistry. At embryonic day (E) 11 and E13, cells expressing GLAST mRNA were present only in the ventricular zone, where GLAST immunoreactivity was associated with most of the cell bodies of neuroepithelial cells. In addition, GLAST immunoreactivity was detected in radial processes running through the mantle and marginal zones. From this characteristic cytology, GLAST-expressing cells at early stages were judged to be radial glia cells. At E15, cells expressing GLAST mRNA first appeared in the mantle zone, and GLAST-immunopositive punctate or reticular protrusions were formed along the radial processes. From E18 to postnatal day (P) 7, GLAST mRNA or its immunoreactivity gradually decreased from the ventricular zone and disappeared from radial processes, whereas cells with GLAST mRNA spread all over the mantle zone and GLAST-immunopositive punctate/reticular protrusions predominated in the neuropils. At P7, GLAST-expressing cells were immunopositive for glial fibrillary acidic protein, an intermediate filament specific to astrocytes. Therefore, the glutamate transporter GLAST is expressed from radial glia through astrocytes during spinal cord development. Furthermore, the distinct changes in the cell position and morphology suggest that both the migration and transformation of radial glia cells begin in the spinal cord between E13 and E15, when the active stage of neuronal migration is over.
Relation: http://www.jneurosci.org/content/17/23/9212.full.pdf+html
Type: article
URI: http://hdl.handle.net/2115/51749
Appears in Collections:医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 渡邉 雅彦

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