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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Medicine / Faculty of Medicine >
Peer-reviewed Journal Articles, etc >
Distal extension of climbing fiber territory and multiple innervation caused by aberrant wiring to adjacent spiny branchlets in cerebellar Purkinje cells lacking glutamate receptor GluRδ2
| Title: | Distal extension of climbing fiber territory and multiple innervation caused by aberrant wiring to adjacent spiny branchlets in cerebellar Purkinje cells lacking glutamate receptor GluRδ2 |
| Authors: | Ichikawa, Ryoichi Browse this author | | Miyazaki, Taisuke Browse this author →KAKEN DB | | Kano, Masanobu Browse this author | | Hashikawa, Tsutomu Browse this author | | Tatsumi, Haruyuki Browse this author | | Sakimura, Kenji Browse this author | | Mishina, Masayoshi Browse this author | | Inoue, Yoshiro Browse this author →KAKEN DB | | Watanabe, Masahiko Browse this author →KAKEN DB |
| Issue Date: | 1-Oct-2002 |
| Publisher: | Society for Neuroscience |
| Journal Title: | The Journal of neuroscience : the official journal of the Society for Neuroscience |
| Volume: | 22 |
| Issue: | 19 |
| Start Page: | 8487 |
| End Page: | 8503 |
| Publisher DOI: | 10.1523/JNEUROSCI.22-19-08487.2002 |
| PMID: | 12351723 |
| Abstract: | Organized synapse formation on to Purkinje cell (PC) dendrites by parallel fibers (PFs) and climbing fibers (CFs) is crucial for cerebellar function. In PCs lacking glutamate receptor delta2 (GluRdelta2), PF synapses are reduced in number, numerous free spines emerge, and multiple CF innervation persists to adulthood. In the present study, we conducted anterograde and immunohistochemical labelings to investigate how CFs innervate PC dendrites under weakened synaptogenesis by PFs. In the GluRdelta2 knock-out mouse, CFs were distributed in the molecular layer more closely to the pial surface compared with the wild-type mouse. Serial electron microscopy demonstrated that CFs in the knock-out mouse innervated all spines protruding from proximal dendrites of PCs, as did those in the wild-type mouse. In the knock-out mouse, however, CF innervation extended distally to spiny branchlets, where nearly half of the spines were free of innervation in contrast to complete synapse formation by PFs in the wild-type mouse. Furthermore, from the end point of innervation, CFs aberrantly jumped to form ectopic synapses on adjacent spiny branchlets, whose proximal portions were often innervated by different CFs. Without GluRdelta2, CFs are thus able to expand their territory along and beyond dendritic trees of the target PC, resulting in persistent surplus CFs by innervating the distal dendritic segment. We conclude that GluRdelta2 is essential to restrict CF innervation to the proximal dendritic segment, by which territorized innervation by PFs and CFs is properly structured and the formation of excess CF wiring to adjacent PCs is suppressed. |
| Relation: | http://www.jneurosci.org/content/22/19/8487.full.pdf+html |
| Type: | article |
| URI: | http://hdl.handle.net/2115/51753 |
| Appears in Collections: | 医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 渡邉 雅彦
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