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P/Q-type Ca2+ channel alpha1A regulates synaptic competition on developing cerebellar Purkinje cells.

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Title: P/Q-type Ca2+ channel alpha1A regulates synaptic competition on developing cerebellar Purkinje cells.
Authors: Miyazaki, Taisuke Browse this author →KAKEN DB
Hashimoto, Kouichi Browse this author
Shin, Hee-Sup Browse this author
Kano, Masanobu Browse this author
Watanabe, Masahiko Browse this author →KAKEN DB
Keywords: Cerebellum
Purkinje cell
Climbing fiber
Parallel fiber
P/Q-type calcium channel
P/Q-type calcium channel
Alpha1A subunit
Synapse formation
Issue Date: 18-Feb-2004
Publisher: Society for Neuroscience
Journal Title: The Journal of neuroscience : the official journal of the Society for Neuroscience
Volume: 24
Issue: 7
Start Page: 1734
End Page: 1743
Publisher DOI: 10.1523/JNEUROSCI.4208-03.2004
PMID: 14973254
Abstract: Synapse formation depends critically on the competition among inputs of multiple sources to individual neurons. Cerebellar Purkinje cells have highly organized synaptic wiring from two distinct sources of excitatory afferents. Single climbing fibers innervate proximal dendrites of Purkinje cells, whereas numerous parallel fibers converge on their distal dendrites. Here, we demonstrate that the P/Q-type Ca2+ channel alpha1A, a major Ca2+ channel subtype in Purkinje cells, is crucial for this organized synapse formation. In the alpha1A knock-out mouse, many ectopic spines were protruded from proximal dendrites and somata of Purkinje cells. Innervation territory of parallel fibers was expanded proximally to innervate the ectopic spines, whereas that of climbing fibers was regressed to the basal portion of proximal dendrites and somata. Furthermore, multiple climbing fibers consisting of a strong climbing fiber and one or a few weaker climbing fibers, persisted in the majority of Purkinje cells and were cowired to the same somata, proximal dendrites, or both. Therefore, the lack of alpha1A results in the persistence of parallel fibers and surplus climbing fibers, which should normally be expelled from the compartment innervated by the main climbing fiber. These results suggest that a P/Q-type Ca2+ channel alpha1A fuels heterosynaptic competition between climbing fibers and parallel fibers and also fuels homosynaptic competition among multiple climbing fibers. This molecular function facilitates the distal extension of climbing fiber innervation along the dendritic tree of the Purkinje cell and also establishes climbing fiber monoinnervation of individual Purkinje cells.
Type: article
Appears in Collections:医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 渡邉 雅彦

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