HUSCAP logo Hokkaido Univ. logo

Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Agriculture / Faculty of Agriculture >
Peer-reviewed Journal Articles, etc >

A Novel Metabolic Pathway for Glucose Production Mediated by α-Glucosidase-catalyzed Conversion of 1,5-Anhydrofructose

Files in This Item:
JBC287-27_22441-22444.pdf2.7 MBPDFView/Open
Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/51767

Title: A Novel Metabolic Pathway for Glucose Production Mediated by α-Glucosidase-catalyzed Conversion of 1,5-Anhydrofructose
Authors: Kim, Young-Min Browse this author
Saburi, Wataru Browse this author →KAKEN DB
Yu, Shukun Browse this author
Nakai, Hiroyuki Browse this author
Maneesan, Janjira Browse this author
Kang, Min-Sun Browse this author
Chiba, Seiya Browse this author →KAKEN DB
Kim, Doman Browse this author
Okuyama, Masayuki Browse this author →KAKEN DB
Mori, Haruhide Browse this author →KAKEN DB
Kimura, Atsuo Browse this author →KAKEN DB
Keywords: α-glucosidase
hydration
trans-addition
1,5-anhydrofructose
Issue Date: 29-Jun-2012
Publisher: American Society for Biochemistry and Molecular Biology
Journal Title: Journal of Biological Chemistry
Volume: 287
Issue: 27
Start Page: 22441
End Page: 22444
Publisher DOI: 10.1074/jbc.C112.360909
Abstract: α-Glucosidase is in the glycoside hydrolase family 13 (13AG) and 31 (31AG). Only 31AGs can hydrate the D-glucal double bond to form α-2-deoxyglucose. Since 1,5-anhydrofructose (AF), having 2-OH-group, mimics the oxocarbenium-ion transition-state, AF may be a substrate for α-glucosidases. α-Glucosidase-catalyzed hydration produced α-glucose from AF, which plateaued with time. Combined reaction with α-1,4-glucan lyase (GL) and 13AG eliminated the plateau. Aspergillus niger α-glucosidase (31AG), which is stable in organic solvent, produced ethyl α-glucoside from AF in 80% ethanol. The findings indicate that α-glucosidases catalyze trans-addition. This is the first report of α-glucosidase-associated glucose formation from AF, possibly contributing to salvage pathway of un-utilized AF.
Rights: This research was originally published in Journal of Biological Chemistry. Young-Min Kim; Wataru Saburi; Shukun Yu; Hiroyuki Nakai; Janjira Maneesan; Min-Sun Kang; Seiya Chiba; Doman Kim; Masayuki Okuyama; Haruhide Mori; Atsuo Kimura. A Novel Metabolic Pathway for Glucose Production Mediated by α-Glucosidase-catalyzed Conversion of 1,5-Anhydrofructose. Journal of Biological Chemistry. 2012; 287:22441-22444. © the American Society for Biochemistry and Molecular Biology.
Type: article (author version)
URI: http://hdl.handle.net/2115/51767
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 木村 淳夫

Export metadata:

OAI-PMH ( junii2 , jpcoar )


 

Feedback - Hokkaido University