Title: | A Novel Metabolic Pathway for Glucose Production Mediated by α-Glucosidase-catalyzed Conversion of 1,5-Anhydrofructose |
Authors: | Kim, Young-Min Browse this author |
Saburi, Wataru Browse this author →KAKEN DB |
Yu, Shukun Browse this author |
Nakai, Hiroyuki Browse this author |
Maneesan, Janjira Browse this author |
Kang, Min-Sun Browse this author |
Chiba, Seiya Browse this author →KAKEN DB |
Kim, Doman Browse this author |
Okuyama, Masayuki Browse this author →KAKEN DB |
Mori, Haruhide Browse this author →KAKEN DB |
Kimura, Atsuo Browse this author →KAKEN DB |
Keywords: | α-glucosidase |
hydration |
trans-addition |
1,5-anhydrofructose |
Issue Date: | 29-Jun-2012 |
Publisher: | American Society for Biochemistry and Molecular Biology |
Journal Title: | Journal of Biological Chemistry |
Volume: | 287 |
Issue: | 27 |
Start Page: | 22441 |
End Page: | 22444 |
Publisher DOI: | 10.1074/jbc.C112.360909 |
Abstract: | α-Glucosidase is in the glycoside hydrolase family 13 (13AG) and 31 (31AG). Only 31AGs can hydrate the D-glucal double bond to form α-2-deoxyglucose. Since 1,5-anhydrofructose (AF), having 2-OH-group, mimics the oxocarbenium-ion transition-state, AF may be a substrate for α-glucosidases. α-Glucosidase-catalyzed hydration produced α-glucose from AF, which plateaued with time. Combined reaction with α-1,4-glucan lyase (GL) and 13AG eliminated the plateau. Aspergillus niger α-glucosidase (31AG), which is stable in organic solvent, produced ethyl α-glucoside from AF in 80% ethanol. The findings indicate that α-glucosidases catalyze trans-addition. This is the first report of α-glucosidase-associated glucose formation from AF, possibly contributing to salvage pathway of un-utilized AF. |
Rights: | This research was originally published in Journal of Biological Chemistry. Young-Min Kim; Wataru Saburi; Shukun Yu; Hiroyuki Nakai; Janjira Maneesan; Min-Sun Kang; Seiya Chiba; Doman Kim; Masayuki Okuyama; Haruhide Mori; Atsuo Kimura. A Novel Metabolic Pathway for Glucose Production Mediated by α-Glucosidase-catalyzed Conversion of 1,5-Anhydrofructose. Journal of Biological Chemistry. 2012; 287:22441-22444. © the American Society for Biochemistry and Molecular Biology. |
Type: | article (author version) |
URI: | http://hdl.handle.net/2115/51767 |
Appears in Collections: | 農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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