| Title: | The use of Loop-mediated Isothermal Amplification (LAMP) to detect the re-emerging Human African Trypanosomiasis (HAT) in the Luangwa and Zambezi valleys |
| Authors: | Namangala, Boniface Browse this author |
| Hachaambwa, Lottie Browse this author |
| Kajino, Kiichi Browse this author →KAKEN DB |
| Mweene, Aaron S. Browse this author |
| Hayashida, Kyouko Browse this author |
| Simuunza, Martin Browse this author |
| Simukoko, Humphrey Browse this author |
| Choongo, Kennedy Browse this author |
| Chansa, Pamela Browse this author |
| Lakhi, Shabir Browse this author |
| Moonga, Ladslav Browse this author |
| Chota, Amos Browse this author |
| Ndebe, Joseph Browse this author |
| Nsakashalo-Senkwe, Mutale Browse this author |
| Chizema, Elizabeth Browse this author |
| Kasonka, Lackson Browse this author |
| Sugimoto, Chihiro Browse this author →KAKEN DB |
| Keywords: | HAT |
| RIME-LAMP |
| SRA-LAMP |
| Trypanosoma brucei rhodesiense |
| Zambia |
| Zimbabwe |
| Issue Date: | 4-Dec-2012 |
| Publisher: | BioMed Central |
| Journal Title: | Parasites & Vectors |
| Volume: | 5 |
| Start Page: | 282 |
| Publisher DOI: | 10.1186/1756-3305-5-282 |
| Abstract: | Background: Loop-mediated isothermal amplification (LAMP) is a novel strategy which amplifies DNA with high sensitivity and rapidity under isothermal conditions. In the present study, the performance of the repetitive insertion mobile element (RIME)-LAMP and human serum resistance-associated gene (SRA)-LAMP assays were evaluated using clinical specimens obtained from four male patients from Luangwa and Zambezi valleys in Zambia and Zimbabwe, respectively. Findings: The cases reported in this preliminary communication were all first diagnosed by microscopy, through passive surveillance, and confirmed by both RIME-LAMP and SRA-LAMP. A good correlation between microscopy and LAMP was observed and contributed to staging and successful treatment of patient. RIME-LAMP and SRA-LAMP complimented each other well in all the cases. Conclusions: Both RIME-LAMP and SRA-LAMP were able to detect Trypanosoma brucei rhodesiense DNA in patient blood and CSF and hence confirmed HAT in the parasitaemic patients. Our study indicates that the LAMP technique is a potential tool for HAT diagnosis, staging and may be useful for making therapeutic decisions. However, no statistically significant conclusion may be drawn due to the limited sample size used in the present study. It is thus imperative to conduct a detailed study to further evaluate the potential of LAMP as a bedside diagnostic test for HAT. |
| Rights: | http://creativecommons.org/licenses/by/2.0/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/51773 |
| Appears in Collections: | 人獣共通感染症国際共同研究所 (International Institute for Zoonosis Control) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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