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The use of Loop-mediated Isothermal Amplification (LAMP) to detect the re-emerging Human African Trypanosomiasis (HAT) in the Luangwa and Zambezi valleys

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Title: The use of Loop-mediated Isothermal Amplification (LAMP) to detect the re-emerging Human African Trypanosomiasis (HAT) in the Luangwa and Zambezi valleys
Authors: Namangala, Boniface Browse this author
Hachaambwa, Lottie Browse this author
Kajino, Kiichi Browse this author →KAKEN DB
Mweene, Aaron S. Browse this author
Hayashida, Kyouko Browse this author
Simuunza, Martin Browse this author
Simukoko, Humphrey Browse this author
Choongo, Kennedy Browse this author
Chansa, Pamela Browse this author
Lakhi, Shabir Browse this author
Moonga, Ladslav Browse this author
Chota, Amos Browse this author
Ndebe, Joseph Browse this author
Nsakashalo-Senkwe, Mutale Browse this author
Chizema, Elizabeth Browse this author
Kasonka, Lackson Browse this author
Sugimoto, Chihiro Browse this author →KAKEN DB
Keywords: HAT
RIME-LAMP
SRA-LAMP
Trypanosoma brucei rhodesiense
Zambia
Zimbabwe
Issue Date: 4-Dec-2012
Publisher: BioMed Central
Journal Title: Parasites & Vectors
Volume: 5
Start Page: 282
Publisher DOI: 10.1186/1756-3305-5-282
Abstract: Background: Loop-mediated isothermal amplification (LAMP) is a novel strategy which amplifies DNA with high sensitivity and rapidity under isothermal conditions. In the present study, the performance of the repetitive insertion mobile element (RIME)-LAMP and human serum resistance-associated gene (SRA)-LAMP assays were evaluated using clinical specimens obtained from four male patients from Luangwa and Zambezi valleys in Zambia and Zimbabwe, respectively. Findings: The cases reported in this preliminary communication were all first diagnosed by microscopy, through passive surveillance, and confirmed by both RIME-LAMP and SRA-LAMP. A good correlation between microscopy and LAMP was observed and contributed to staging and successful treatment of patient. RIME-LAMP and SRA-LAMP complimented each other well in all the cases. Conclusions: Both RIME-LAMP and SRA-LAMP were able to detect Trypanosoma brucei rhodesiense DNA in patient blood and CSF and hence confirmed HAT in the parasitaemic patients. Our study indicates that the LAMP technique is a potential tool for HAT diagnosis, staging and may be useful for making therapeutic decisions. However, no statistically significant conclusion may be drawn due to the limited sample size used in the present study. It is thus imperative to conduct a detailed study to further evaluate the potential of LAMP as a bedside diagnostic test for HAT.
Rights: http://creativecommons.org/licenses/by/2.0/
Type: article
URI: http://hdl.handle.net/2115/51773
Appears in Collections:人獣共通感染症リサーチセンター (Research Center for Zoonosis Control) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 杉本 千尋

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