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Developmental switching of perisomatic innervation from climbing fibers to basket cell fibers in cerebellar Purkinje cells.

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Title: Developmental switching of perisomatic innervation from climbing fibers to basket cell fibers in cerebellar Purkinje cells.
Authors: Ichikawa, Ryoichi Browse this author
Yamasaki, Miwako Browse this author →KAKEN DB
Miyazaki, Taisuke Browse this author →KAKEN DB
Konno, Kohtarou Browse this author
Hashimoto, Kouichi Browse this author
Tatsumi, Haruyuki Browse this author
Inoue, Yoshiro Browse this author →KAKEN DB
Kano, Masanobu Browse this author
Watanabe, Masahiko Browse this author
Issue Date: 23-Nov-2011
Publisher: Society for Neuroscience
Journal Title: The Journal of neuroscience : the official journal of the Society for Neuroscience
Volume: 31
Issue: 47
Start Page: 16916
End Page: 16927
Publisher DOI: 10.1523/JNEUROSCI.2396-11.2011
PMID: 22114262
Abstract: In early postnatal development, perisomatic innervation of cerebellar Purkinje cells (PCs) switches from glutamatergic climbing fibers (CFs) to GABAergic basket cell fibers (BFs). Here we examined the switching process in C57BL/6 mice. At postnatal day 7 (P7), most perisomatic synapses were formed by CFs on to somatic spines. The density of CF-spine synapses peaked at P9, when pericellular nest around PCs by CFs was most developed, and CF-spine synapses constituted 88% of the total perisomatic synapses. Thereafter, CF-spine synapses dropped to 63% at P12, 6% at P15, and <1% at P20, whereas BF synapses increased reciprocally. During the switching period, a substantial number of BF synapses existed as BF-spine synapses (37% of the total perisomatic synapses at P15), and free spines surrounded by BFs or Bergmann glia also emerged. By P20, BF-spine synapses and free spines virtually disappeared, and BF-soma synapses became predominant (88%), thus attaining the adult pattern of perisomatic innervation. Parallel with the presynaptic switching, postsynaptic receptor phenotype also switched from glutamatergic to GABAergic. In the active switching period, particularly at P12, fragmental clusters of AMPA-type glutamate receptor were juxtaposed with those of GABA(A) receptor. When examined with serial ultrathin sections, immunogold labeling for glutamate and GABA(A) receptors was often clustered beneath single BF terminals. These results suggest that a considerable fraction of somatic spines is succeeded from CFs to BFs and Bergmann glia in the early postnatal period, and that the switching of postsynaptic receptor phenotypes mainly proceeds under the coverage of BF terminals.
Type: article
Appears in Collections:医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 渡邉 雅彦

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