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Identification of proteins involved in membrane fouling in membrane bioreactors (MBRs) treating municipal wastewater

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Title: Identification of proteins involved in membrane fouling in membrane bioreactors (MBRs) treating municipal wastewater
Authors: Miyoshi, Taro Browse this author
Aizawa, Tomoyasu Browse this author →KAKEN DB
Kimura, Katsuki Browse this author →KAKEN DB
Watanabe, Yoshimasa Browse this author →KAKEN DB
Keywords: Membrane bioreactor
Membrane fouling
Foulant characterization
Issue Date: Nov-2012
Publisher: Elsevier
Journal Title: International Biodeterioration & Biodegradation
Volume: 75
Start Page: 15
End Page: 22
Publisher DOI: 10.1016/j.ibiod.2012.08.003
Abstract: The proteins that caused membrane fouling in a continuous operation of MBRs treating real municipal wastewater were investigated in detail. We continuously operated two identical pilot-scale MBRs under different solid retention times (SRTs) and extracted the foulants at the end of the operation. Regardless of the operating conditions, proteins were dominant components in the foulants extracted from the fouled membranes. The extracted proteins were subjected to the separation with two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and the identification through the N-terminal amino acid sequencing analysis. The proteins concentrated by the combination of the crude concentration using an ultra-filtration (UF) membrane and trichloroacetic acid (TCA) precipitation were separated and visualized well on 2D-PAGE gels. The results of 2D-PAGE analysis indicated that the compositions of proteins that caused membrane fouling significantly differed depending on the SRT, although such differences cannot be seen in the amino acid composition analysis. Analyzing selected 2D-PAGE spots by N-terminal amino acid sequencing analysis led to the identification of two well-characterized outer membrane proteins originating from Pseudomonas genus, namely OprF and OprD. To our knowledge, this is the first successful identification of proteins that have caused membrane fouling in continuous operations of MBRs treating real wastewater.
Type: article (author version)
Appears in Collections:環境ナノ・バイオ工学研究センター (Research Center for Environmental Nano and Bio Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 三好 太郎

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