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Rapid species identification of fresh and processed scallops by multiplex PCR

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Title: Rapid species identification of fresh and processed scallops by multiplex PCR
Authors: Marín, Alan Browse this author
Fujimoto, Takafumi Browse this author
Arai, Katsutoshi Browse this author →KAKEN DB
Keywords: Pectinidae
Food control
16S rRNA gene
Multiplex PCR
Issue Date: Aug-2013
Publisher: Elsevier
Journal Title: Food control
Volume: 32
Issue: 2
Start Page: 472
End Page: 476
Publisher DOI: 10.1016/j.foodcont.2013.01.007
Abstract: Food control policies regarding to seafood label authenticity have become a global issue due to increased incidence of species substitution or mislabelling. Proper species-level identification in processed scallop products is hindered by the lack of morphological characters such as their valves. In order to identify four commercially important scallop species (Argopecten purpuratus, A. irradians, Mizuhopecten yessoensis, Pecten albicans) a species-specific multiplex PCR reaction is described herein. Novel reverse species-specific primers in combination with one universal forward primer designed to amplify a partial region of the mitochondrial 16S rRNA gene were assayed in fresh as well as in manufactured scallop samples. All PCR reactions showed a high specificity allowing an unambiguous species authentication.
Type: article (author version)
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 荒井 克俊

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